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GeneTex
United States (US)

PFKL antibody [C1C3]

GTX105697_40023_20150417_IHC_M_19011506_828.jpg
GTX105697_WB_19011506_854.jpg
GTX105697_40023_IHC_19011506_255.jpg
GTX105697_IFA_19011506_473.jpg

Cat No. GTX105697

Reference 1
Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
See all PFKL products
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

85 kDa. ( Note )

Positive Control

A431 , H1299 , HeLa , HepG2

Predict Reactivity

Rat, Bovine, Chicken(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.8 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PFKL. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Phosphofructokinase, Liver Type,Atp-Pfk,Pfk-B,Pfk-L,Pfkl

Background

Phosphofructokinase (PFK) is a tetrameric enzyme that catalyzes a key step in glycolysis, namely the conversion of D-fructose 6-phosphate to D-fructose 1,6-bisphosphate. Separate genes encode a muscle subunit (M) and a liver subunit (L). PFK from muscle is a homotetramer of M subunits, PFK from liver is a homotetramer of L-subunits, while PFK from platelets can be composed of any tetrameric combination of M and L subunits. The protein encoded by this gene represents the L subunit. Alternate splicing results in two transcript variants, one of which is a candidate for nonsense-mediated decay (NMD). [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX105697_40023_20150417_IHC_M_19011506_828.jpg

GTX105697 IHC-P Image

PFKL antibody [C1C3] detects PFKL protein at cytoplasm in mouse cervix by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cervix.
PFKL antibody [C1C3] (GTX105697) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX105697_WB_19011506_854.jpg

GTX105697 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
7.5% SDS PAGE
GTX105697 diluted at 1:1000
GTX105697_40023_IHC_19011506_255.jpg

GTX105697 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using PFKL(GTX105697) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX105697_IFA_19011506_473.jpg

GTX105697 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A549, using PFKL(GTX105697) antibody at 1:200 dilution.
REFERENCE

Application Reference

Hu CM et al. Cell Metab 2019; (Epub ahead of print) High Glucose Triggers Nucleotide Imbalance through O-GlcNAcylation of Key Enzymes and Induces KRAS Mutation in Pancreatic Cells.
Application : WB
Reactivity : Human
Submit a Reference
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169