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PFKL antibody [C1C3]

Anti-PFKL antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX105697
Anti-PFKL antibody [C1C3] used in Western Blot (WB). GTX105697
Anti-PFKL antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX105697
Anti-PFKL antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105697

Cat No. GTX105697

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($329),
25 μl ($169)
See all PFKL products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

85 kDa. ( Note )

Positive Control

A431 , H1299 , HeLa , HepG2

Predict Reactivity

Rat, Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.8 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PFKL. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951188

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

phosphofructokinase, liver type , ATP-PFK , PFK-B , PFK-L

Background

Phosphofructokinase (PFK) is a tetrameric enzyme that catalyzes a key step in glycolysis, namely the conversion of D-fructose 6-phosphate to D-fructose 1,6-bisphosphate. Separate genes encode a muscle subunit (M) and a liver subunit (L). PFK from muscle is a homotetramer of M subunits, PFK from liver is a homotetramer of L-subunits, while PFK from platelets can be composed of any tetrameric combination of M and L subunits. The protein encoded by this gene represents the L subunit. Alternate splicing results in two transcript variants, one of which is a candidate for nonsense-mediated decay (NMD). [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PFKL antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX105697

GTX105697 IHC-P Image

PFKL antibody [C1C3] detects PFKL protein at cytoplasm in mouse cervix by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cervix.
PFKL antibody [C1C3] (GTX105697) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PFKL antibody [C1C3] used in Western Blot (WB). GTX105697

GTX105697 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
7.5% SDS PAGE
GTX105697 diluted at 1:1000

Anti-PFKL antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX105697

GTX105697 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using PFKL(GTX105697) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PFKL antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105697

GTX105697 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A549, using PFKL(GTX105697) antibody at 1:200 dilution.

REFERENCE

Application Reference

Hu CM et al. Cell Metab 2019; (Epub) High Glucose Triggers Nucleotide Imbalance through O-GlcNAcylation of Key Enzymes and Induces KRAS Mutation in Pancreatic Cells.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169