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PFKL antibody [C1C3]

Overexpression
Anti-PFKL antibody [C1C3] used in Western Blot (WB). GTX105697
Anti-PFKL antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX105697
Anti-PFKL antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX105697
Anti-PFKL antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105697

Cat. No. GTX105697

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all PFKL products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

85 kDa. ( Note )

Positive Control

HepG2

Product Note

Based on sequence homology, this antibody may cross react with PFKP or PFKM protein.

Predict Reactivity

Rat, Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.23 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PFKL. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951188

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

phosphofructokinase, liver type , ATP-PFK , PFK-B , PFK-L

Background

Phosphofructokinase (PFK) is a tetrameric enzyme that catalyzes a key step in glycolysis, namely the conversion of D-fructose 6-phosphate to D-fructose 1,6-bisphosphate. Separate genes encode a muscle subunit (M) and a liver subunit (L). PFK from muscle is a homotetramer of M subunits, PFK from liver is a homotetramer of L-subunits, while PFK from platelets can be composed of any tetrameric combination of M and L subunits. The protein encoded by this gene represents the L subunit. Alternate splicing results in two transcript variants, one of which is a candidate for nonsense-mediated decay (NMD). [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PFKL antibody [C1C3] used in Western Blot (WB). GTX105697

GTX105697 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PFKL antibody [C1C3] (GTX105697) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PFKL antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX105697

GTX105697 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using PFKL(GTX105697) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PFKL antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX105697

GTX105697 IHC-P Image

PFKL antibody [C1C3] detects PFKL protein at cytoplasm in mouse cervix by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cervix.
PFKL antibody [C1C3] (GTX105697) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PFKL antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105697

GTX105697 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A549, using PFKL(GTX105697) antibody at 1:200 dilution.

REFERENCE

Application Reference

Wang C et al. Nat Commun 2023; 14 (1) : 959 The KLF7/PFKL/ACADL axis modulates cardiac metabolic remodelling during cardiac hypertrophy in male mice.
Application : WB
Reactivity : Mouse
Hu CM et al. Cell Metab 2019; (Epub) High Glucose Triggers Nucleotide Imbalance through O-GlcNAcylation of Key Enzymes and Induces KRAS Mutation in Pancreatic Cells.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169