APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Dilution |
1:500-1:3000 |
1:100-1:1000 |
1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
A431 , H1299 , HeLa , HepG2
Predict Reactivity
Rat, Bovine, Chicken(>80% identity)
PROPERTIES
Form
Liquid
Buffer
0.1M Tris-Glycine (pH7), 10% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.8 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human PFKL. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1951188
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
phosphofructokinase, liver type , ATP-PFK , PFK-B , PFK-L
Background
Phosphofructokinase (PFK) is a tetrameric enzyme that catalyzes a key step in glycolysis, namely the conversion of D-fructose 6-phosphate to D-fructose 1,6-bisphosphate. Separate genes encode a muscle subunit (M) and a liver subunit (L). PFK from muscle is a homotetramer of M subunits, PFK from liver is a homotetramer of L-subunits, while PFK from platelets can be composed of any tetrameric combination of M and L subunits. The protein encoded by this gene represents the L subunit. Alternate splicing results in two transcript variants, one of which is a candidate for nonsense-mediated decay (NMD). [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX105697 IHC-P Image
PFKL antibody [C1C3] detects PFKL protein at cytoplasm in mouse cervix by immunohistochemical analysis. Sample: Paraffin-embedded mouse cervix. PFKL antibody [C1C3] (GTX105697) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX105697 WB Image
Sample (30 ug of whole cell lysate) A: Hep G2 (GTX27900) 7.5% SDS PAGE GTX105697 diluted at 1:1000
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GTX105697 IHC-P Image
Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using PFKL(GTX105697) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX105697 ICC/IF Image
Immunofluorescence analysis of paraformaldehyde-fixed A549, using PFKL(GTX105697) antibody at 1:200 dilution.
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REFERENCE
REVIEW
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