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PFKM antibody [AT2F11]

Anti-PFKM antibody [AT2F11] used in Western Blot (WB). GTX53761

Cat. No. GTX53761

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 AT2F11

Isotype

 IgG2a

Application

 WB, ELISA

Reactivity

 Human
Package
100 μl ($399)
See all PFKM products

APPLICATION

Application Note

The antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 1:2000. Recommended starting dilution is 1:2000.

Calculated MW

85 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human Fructose 6 Phosphate Kinase (1-780aa) purified from E. coli

Purification

By protein-A affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

phosphofructokinase, muscle , ATP-PFK , GSD7 , PFK-1 , PFK-A , PFK1 , PFKA , PFKX , PPP1R122

Cellular Localization

Cytoplasm

Background

Three phosphofructokinase isozymes exist in humans: muscle, liver and platelet. These isozymes function as subunits of the mammalian tetramer phosphofructokinase, which catalyzes the phosphorylation of fructose-6-phosphate to fructose-1,6-bisphosphate. Tetramer composition varies depending on tissue type. This gene encodes the muscle-type isozyme. Mutations in this gene have been associated with glycogen storage disease type VII, also known as Tarui disease. Alternatively spliced transcript variants have been described.[provided by RefSeq, Nov 2009]

Database

Research Area

DATA IMAGES

Anti-PFKM antibody [AT2F11] used in Western Blot (WB). GTX53761

GTX53761 WB Image

WB analysis of HepG2 lysate (30ug) using PFKM antibody [AT2F11] at the indicated dilutions.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399