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PFKM antibody [C1C3]

KO/KD
Anti-PFKM antibody [C1C3] used in Western Blot (WB). GTX111597
Anti-PFKM antibody [C1C3] used in Western Blot (WB). GTX111597
Anti-PFKM antibody [C1C3] used in Western Blot (WB). GTX111597
Anti-PFKM antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111597
Anti-PFKM antibody [C1C3] used in Western Blot (WB). GTX111597
Anti-PFKM antibody [C1C3] used in Western Blot (WB). GTX111597

Cat. No. GTX111597

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all PFKM products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

85 kDa. ( Note )

Positive Control

A431 , H1299 , mouse brain , rat brain , 293T , HeLa

Predict Reactivity

Bovine, Chicken, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.57 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Fructose 6 Phosphate Kinase. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951192

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

phosphofructokinase, muscle , ATP-PFK , GSD7 , PFK-1 , PFK-A , PFK1 , PFKA , PFKX , PPP1R122

Background

The PFKM gene encodes the muscle isoform of phosphofructokinase (PFK) (ATP:D-fructose-6-phosphate-1-phosphotransferase, EC 2.7.1.11). PFK catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate and is a key regulatory enzyme in glycolysis. Mammalian PFK is a tetramer made up of various combinations of 3 subunits: muscle (PFKM), liver (PFKL; MIM 171860), and platelet (PFKP; MIM 171840), the genes for which are located on chromosomes 12q13, 21q22, and 10p, respectively. The composition of the tetramers differs according to the tissue type. Muscle and liver PFK are a homotetramers of 4M and 4L subunits, respectively. Erythrocytes contain both L and M subunits, which randomly tetramerize to form M4, L4, and M3L, M2L2, and ML3 hybrid forms of the holoenzyme (Vora et al., 1980 [PubMed 6444721]; Raben and Sherman, 1995 [PubMed 7550225]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-PFKM antibody [C1C3] used in Western Blot (WB). GTX111597

GTX111597 WB Image

KO/KD

Wild-type (WT) and PFKM knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PFKM antibody [C1C3] (GTX111597) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PFKM antibody [C1C3] used in Western Blot (WB). GTX111597

GTX111597 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PFKM antibody [C1C3] (GTX111597) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-PFKM antibody [C1C3] used in Western Blot (WB). GTX111597

GTX111597 WB Image

Sample (30 μg of whole cell lysate)
A: A431 (GTX27909)
B: H1299
7.5% SDS PAGE
GTX111597 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PFKM antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111597

GTX111597 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using PFKM antibody [C1C3] (GTX111597) antibody at 1:200 dilution.

Anti-PFKM antibody [C1C3] used in Western Blot (WB). GTX111597

GTX111597 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PFKM antibody [C1C3] (GTX111597) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PFKM antibody [C1C3] used in Western Blot (WB). GTX111597

GTX111597 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PFKM antibody [C1C3] (GTX111597) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Hu CM et al. Cell Metab 2019; (Epub) High Glucose Triggers Nucleotide Imbalance through O-GlcNAcylation of Key Enzymes and Induces KRAS Mutation in Pancreatic Cells.
Application : WB
Reactivity : Human
Mendez-Lucas A et al. Cancer Res 2017; Glucose catabolism in liver tumors induced by c-MYC can be sustained by various PKM1/PKM2 ratios and pyruvate kinase activities.
Application : WB
Reactivity : Mouse
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169