GeneTex

  • Country / Location Selection

United States (US)

PFKM antibody [HL2010]

Recombinant antibody KO/KD Orthogonal
Anti-PFKM antibody [HL2010] used in Western Blot (WB). GTX637911
Anti-PFKM antibody [HL2010] used in Western Blot (WB). GTX637911
Anti-PFKM antibody [HL2010] used in IHC (Paraffin sections) (IHC-P). GTX637911
Anti-PFKM antibody [HL2010] used in IHC (Paraffin sections) (IHC-P). GTX637911
Anti-PFKM antibody [HL2010] used in Western Blot (WB). GTX637911

Cat. No. GTX637911

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2010

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all PFKM products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

85 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human PFKM

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

phosphofructokinase, muscle , ATP-PFK , GSD7 , PFK-1 , PFK-A , PFK1 , PFKA , PFKX , PPP1R122

Cellular Localization

Cytoplasm

Background

Three phosphofructokinase isozymes exist in humans: muscle, liver and platelet. These isozymes function as subunits of the mammalian tetramer phosphofructokinase, which catalyzes the phosphorylation of fructose-6-phosphate to fructose-1,6-bisphosphate. Tetramer composition varies depending on tissue type. This gene encodes the muscle-type isozyme. Mutations in this gene have been associated with glycogen storage disease type VII, also known as Tarui disease. Alternatively spliced transcript variants have been described.[provided by RefSeq, Nov 2009]

Database

Research Area

DATA IMAGES

Anti-PFKM antibody [HL2010] used in Western Blot (WB). GTX637911

GTX637911 WB Image

KO/KD

Wild-type (WT) and PFKM knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PFKM antibody [HL2010] (GTX637911) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PFKM antibody [HL2010] used in Western Blot (WB). GTX637911

GTX637911 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PFKM antibody [HL2010] (GTX637911) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PFKM antibody [HL2010] used in IHC (Paraffin sections) (IHC-P). GTX637911

GTX637911 IHC-P Image

PFKM antibody [HL2010] detects PFKM protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
PFKM stained by PFKM antibody [HL2010] (GTX637911) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PFKM antibody [HL2010] used in IHC (Paraffin sections) (IHC-P). GTX637911

GTX637911 IHC-P Image

PFKM antibody [HL2010] detects PFKM protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
PFKM stained by PFKM antibody [HL2010] (GTX637911) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PFKM antibody [HL2010] used in Western Blot (WB). GTX637911

GTX637911 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PFKM antibody [HL2010] (GTX637911) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

REFERENCE

There are currently no references for PFKM antibody [HL2010] (GTX637911). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for PFKM antibody [HL2010] (GTX637911). Be the first to share your experience with this product.
Submit a Review
Package List Price ($)
$ 399
$ 169
Custom Conjugations