APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
Not tested in other applications.
Calculated MW
Positive Control
mouse brain , rat brain , HeLa
Predict Reactivity
Bovine, Chicken, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.52 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human Fructose 6 Phosphate Kinase. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1951190
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
phosphofructokinase, muscle , ATP-PFK , GSD7 , PFK-1 , PFK-A , PFK1 , PFKA , PFKX , PPP1R122
Background
The PFKM gene encodes the muscle isoform of phosphofructokinase (PFK) (ATP:D-fructose-6-phosphate-1-phosphotransferase, EC 2.7.1.11). PFK catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate and is a key regulatory enzyme in glycolysis. Mammalian PFK is a tetramer made up of various combinations of 3 subunits: muscle (PFKM), liver (PFKL; MIM 171860), and platelet (PFKP; MIM 171840), the genes for which are located on chromosomes 12q13, 21q22, and 10p, respectively. The composition of the tetramers differs according to the tissue type. Muscle and liver PFK are a homotetramers of 4M and 4L subunits, respectively. Erythrocytes contain both L and M subunits, which randomly tetramerize to form M4, L4, and M3L, M2L2, and ML3 hybrid forms of the holoenzyme (Vora et al., 1980 [PubMed 6444721]; Raben and Sherman, 1995 [PubMed 7550225]).[supplied by OMIM]
Database
Research Area
DATA IMAGES
![Anti-PFKM antibody [N1N2-2], N-term used in Western Blot (WB). GTX111461](/templates/default/js/lightbox/images/loading.gif "Anti-PFKM antibody [N1N2-2], N-term used in Western Blot (WB). GTX111461")
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GTX111461 WB Image
Wild-type (WT) and PFKM knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PFKM antibody [N1N2-2], N-term (GTX111461) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX111461 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PFKM antibody [N1N2-2], N-term (GTX111461) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX111461 WB Image
Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PFKM antibody [N1N2-2], N-term (GTX111461) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
There are currently no references for PFKM antibody [N1N2-2], N-term (GTX111461). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for PFKM antibody [N1N2-2], N-term (GTX111461). Be the first to share your experience with this product.
![Anti-PFKM antibody [N1N2-2], N-term used in Western Blot (WB). GTX111461](/upload/website/prouct_img/small/GTX111461/GTX111461_40065_20221209_WB_KO_watermark_22121123_754.webp "Anti-PFKM antibody [N1N2-2], N-term used in Western Blot (WB). GTX111461")
![Anti-PFKM antibody [N1N2-2], N-term used in Western Blot (WB). GTX111461](/upload/website/prouct_img/small/GTX111461/GTX111461_43838_20221209_WB_22121123_994.webp "Anti-PFKM antibody [N1N2-2], N-term used in Western Blot (WB). GTX111461")
![Anti-PFKM antibody [N1N2-2], N-term used in Western Blot (WB). GTX111461](/upload/website/prouct_img/small/GTX111461/GTX111461_40065_20221209_WB_M_R_22121123_267.webp "Anti-PFKM antibody [N1N2-2], N-term used in Western Blot (WB). GTX111461")
![Anti-PFKM antibody [N1N2-2], N-term used in Western Blot (WB). GTX111461](/upload/website/prouct_img/normal/GTX111461/GTX111461_40065_20221209_WB_KO_watermark_22121123_754.webp "Anti-PFKM antibody [N1N2-2], N-term used in Western Blot (WB). GTX111461")
![Anti-PFKM antibody [N1N2-2], N-term used in Western Blot (WB). GTX111461](/upload/website/prouct_img/normal/GTX111461/GTX111461_43838_20221209_WB_22121123_994.webp "Anti-PFKM antibody [N1N2-2], N-term used in Western Blot (WB). GTX111461")
![Anti-PFKM antibody [N1N2-2], N-term used in Western Blot (WB). GTX111461](/upload/website/prouct_img/normal/GTX111461/GTX111461_40065_20221209_WB_M_R_22121123_267.webp "Anti-PFKM antibody [N1N2-2], N-term used in Western Blot (WB). GTX111461")