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PFKM antibody

Anti-PFKM antibody used in IHC (Paraffin sections) (IHC-P). GTX33405
Anti-PFKM antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33405
Anti-PFKM antibody used in Western Blot (WB). GTX33405
Anti-PFKM antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33405
Anti-PFKM antibody used in IHC (Paraffin sections) (IHC-P). GTX33405
Anti-PFKM antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33405
Anti-PFKM antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33405

Cat. No. GTX33405

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all PFKM products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

85 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 501-780 of human PFKM (NP_001160160.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

phosphofructokinase, muscle , ATP-PFK , GSD7 , PFK-1 , PFK-A , PFK1 , PFKA , PFKX , PPP1R122

Cellular Localization

Cytoplasm

Background

Three phosphofructokinase isozymes exist in humans: muscle, liver and platelet. These isozymes function as subunits of the mammalian tetramer phosphofructokinase, which catalyzes the phosphorylation of fructose-6-phosphate to fructose-1,6-bisphosphate. Tetramer composition varies depending on tissue type. This gene encodes the muscle-type isozyme. Mutations in this gene have been associated with glycogen storage disease type VII, also known as Tarui disease. Alternatively spliced transcript variants have been described.[provided by RefSeq, Nov 2009]

Database

Research Area

DATA IMAGES

Anti-PFKM antibody used in IHC (Paraffin sections) (IHC-P). GTX33405

GTX33405 IHC-P Image

IHC-P analysis of human gastric cancer tissue using GTX33405 PFKM antibody.
Dilution : 1:100

Anti-PFKM antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33405

GTX33405 ICC/IF Image

ICC/IF analysis of L929 cells using GTX33405 PFKM antibody.
Blue : DAPI
Dilution : 1:100

Anti-PFKM antibody used in Western Blot (WB). GTX33405

GTX33405 WB Image

WB analysis of various sample lysates using GTX33405 PFKM antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-PFKM antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33405

GTX33405 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX33405 PFKM antibody.
Blue : DAPI
Dilution : 1:100

Anti-PFKM antibody used in IHC (Paraffin sections) (IHC-P). GTX33405

GTX33405 IHC-P Image

IHC-P analysis of human esophageal cancer tissue using GTX33405 PFKM antibody.
Dilution : 1:100

Anti-PFKM antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33405

GTX33405 ICC/IF Image

ICC/IF analysis of NIH/3T3 cells using GTX33405 PFKM antibody.
Blue : DAPI
Dilution : 1:100

Anti-PFKM antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33405

GTX33405 ICC/IF Image

ICC/IF analysis of C6 cells using GTX33405 PFKM antibody.
Blue : DAPI
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399