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PGAM1 antibody [AT1G4]

Anti-PGAM1 antibody [AT1G4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31959
Anti-PGAM1 antibody [AT1G4] used in Flow cytometry (FACS). GTX31959
Anti-PGAM1 antibody [AT1G4] used in Western Blot (WB). GTX31959

Cat No. GTX31959

Host Mouse
Clonality Monoclonal
Clone Name AT1G4
Isotype IgG2a
Application WB, ICC/IF, FACS, ELISA
Reactivity Human
APPLICATION

Application Note

WB: 1:1000. ICC/IF: 1:100

Calculated MW

29 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

Liquid. In Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% Glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human PGAM1 (1-254aa) purified from E. coli

Purification

By protein-A affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Phosphoglycerate Mutase 1,Hel-S-35,Pgam-B,Pgama,Pgam1

Background

Phosphoglyceric acid mutase (EC 2.7.5.3) is widely distributed in mammalian tissues where it catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway (summary by Chen et al., 1974 [PubMed 4811757]).[supplied by OMIM, Nov 2010]

Database

Research Area

DATA IMAGES
Anti-PGAM1 antibody [AT1G4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31959

GTX31959 ICC/IF Image

ICC/IF analysis of PGAM1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PGAM1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

Anti-PGAM1 antibody [AT1G4] used in Flow cytometry (FACS). GTX31959

GTX31959 FACS Image

Flow cytometry analysis of PGAM1 in HeLa cell line, staining at 2-5ug for 1x10*6 cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).

Anti-PGAM1 antibody [AT1G4] used in Western Blot (WB). GTX31959

GTX31959 WB Image

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PGAM1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1: 293T cell lysate Lane. 2: Jurkat cell lysate Lane. 3: Raji cell lysate Lane. 4: A431 cell lysate Lane. 5: HeLa cell lysate.

Package List Price ($)
$ 319