GeneTex

  • Country / Location Selection

United States (US)

PGAM1 antibody [GT256]

KO/KD
Anti-PGAM1 antibody [GT256] used in Western Blot (WB). GTX629745
Anti-PGAM1 antibody [GT256] used in Western Blot (WB). GTX629745
Anti-PGAM1 antibody [GT256] used in Western Blot (WB). GTX629745
Anti-PGAM1 antibody [GT256] used in Western Blot (WB). GTX629745
Anti-PGAM1 antibody [GT256] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629745

Cat. No. GTX629745

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT256

Isotype

 IgG2a

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all PGAM1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Positive Control

mouse brain , Rat brain lsate/extract , 293T , A431 , HeLa , HepG2

Predict Reactivity

Bovine, Chicken, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PGAM1. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888146

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

phosphoglycerate mutase 1 , HEL-S-35 , PGAM-B , PGAMA

Database

Research Area

DATA IMAGES

Anti-PGAM1 antibody [GT256] used in Western Blot (WB). GTX629745

GTX629745 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with PGAM1 antibody [GT256] (GTX629745) diluted at 1:2000.

Anti-PGAM1 antibody [GT256] used in Western Blot (WB). GTX629745

GTX629745 WB Image

PGAM1 antibody [GT256] detects PGAM1 protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
12 % SDS-PAGE
PGAM1 antibody [GT256] (GTX629745) dilution: 1:1000

Anti-PGAM1 antibody [GT256] used in Western Blot (WB). GTX629745

GTX629745 WB Image

PGAM1 antibody [GT256] detects PGAM1 protein by Western blot analysis.
A. 50 μg Rat brain lsate/extract
12 % SDS-PAGE
PGAM1 antibody [GT256] (GTX629745) dilution: 1:1000

Anti-PGAM1 antibody [GT256] used in Western Blot (WB). GTX629745

GTX629745 WB Image

PGAM1 antibody [GT256] detects PGAM1 protein by Western blot analysis.
A. 50 μg mouse brain lysate/extract
12 % SDS-PAGE
PGAM1 antibody [GT256] (GTX629745) dilution: 1:1000

Anti-PGAM1 antibody [GT256] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629745

GTX629745 ICC/IF Image

PGAM1 antibody [GT256] detects PGAM1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% PFA for 15 min.
Green: PGAM1 protein stained by PGAM1 antibody [GT256] (GTX629745) diluted at 1:100.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

REFERENCE

There are currently no references for PGAM1 antibody [GT256] (GTX629745). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for PGAM1 antibody [GT256] (GTX629745). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations