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PGAM1 antibody [N1C3]

KO/KD
Anti-PGAM1 antibody [N1C3] used in Western Blot (WB). GTX114633
Anti-PGAM1 antibody [N1C3] used in Western Blot (WB). GTX114633
Anti-PGAM1 antibody [N1C3] used in Western Blot (WB). GTX114633

Cat. No. GTX114633

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all PGAM1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Positive Control

Mouse brain , 293T , A431 , Jurkat , Raji

Predict Reactivity

Rat, Bovine, Chicken, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.47 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PGAM1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10634450

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

phosphoglycerate mutase 1 , HEL-S-35 , PGAM-B , PGAMA

Background

Interconversion of 3- and 2-phosphoglycerate with 2,3-bisphosphoglycerate as the primer of the reaction. Can also catalyze the reaction of EC 5.4.2.4 (synthase) and EC 3.1.3.13 (phosphatase), but with a reduced activity.

Database

Research Area

DATA IMAGES

Anti-PGAM1 antibody [N1C3] used in Western Blot (WB). GTX114633

GTX114633 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with PGAM1 antibody [N1C3] (GTX114633) diluted at 1:1000.

Anti-PGAM1 antibody [N1C3] used in Western Blot (WB). GTX114633

GTX114633 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX114633 diluted at 1:1000

Anti-PGAM1 antibody [N1C3] used in Western Blot (WB). GTX114633

GTX114633 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: A431 (GTX27909)
C: JurKat
D: Raji
12% SDS PAGE
GTX114633 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169