GeneTex
United States (US)

PGAM1 antibody [N1C3]

KO/KD
GTX114633_40205_20160825_WB_shRNA_watermark_18121410_218.jpg
GTX114633_40205_WB_M_brain_18121410_526.jpg
GTX114633_WB_18121410_266.jpg

Cat No. GTX114633

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Positive Control

293T , A431 , Jurkat , Raji , mouse brain , 293T

Predict Reactivity

Rat, Bovine, Chicken, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.47mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PGAM1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

PGAM-B antibody, PGAMA antibody, PGAM1 antibody, "phosphoglycerate mutase A, nonmuscle form antibody", phosphoglycerate mutase isozyme B antibody, phosphoglycerate mutase 1 antibody, BPG-dependent PGAM 1 antibody, phosphoglycerate mutase 1 (brain) antibody

Background

Interconversion of 3- and 2-phosphoglycerate with 2,3-bisphosphoglycerate as the primer of the reaction. Can also catalyze the reaction of EC 5.4.2.4 (synthase) and EC 3.1.3.13 (phosphatase), but with a reduced activity.

Database

Research Area

DATA IMAGES
GTX114633_40205_20160825_WB_shRNA_watermark_18121410_218.jpg

GTX114633 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with PGAM1 antibody [N1C3] (GTX114633) diluted at 1:1000.

GTX114633_40205_WB_M_brain_18121410_526.jpg

GTX114633 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX114633 diluted at 1:1000

GTX114633_WB_18121410_266.jpg

GTX114633 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: A431 (GTX27909)
C: JurKat
D: Raji
12% SDS PAGE
GTX114633 diluted at 1:1000

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169