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PGAM5 antibody

Anti-PGAM5 antibody used in Western Blot (WB). GTX119887
Anti-PGAM5 antibody used in Western Blot (WB). GTX119887

Cat No. GTX119887

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Positive Control

293T , HepG2

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.62 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PGAM5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885454

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

PGAM family member 5, mitochondrial serine/threonine protein phosphatase , BXLBV68

Cellular Localization

Isoform 1: Nucleus , Cytoplasm , Isoform 2: Cytoplasm

Database

Research Area

DATA IMAGES

Anti-PGAM5 antibody used in Western Blot (WB). GTX119887

GTX119887 WB Image

Wild-type (WT) and PGAM5 knockout (KO) 293T cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with PGAM5 antibody (GTX119887) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-PGAM5 antibody used in Western Blot (WB). GTX119887

GTX119887 WB Image

PGAM5 antibody detects PGAM5 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with PGAM5 antibody (GTX119887) diluted by 1:1000.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf