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PGC1 alpha antibody, Internal

Orthogonal
Anti-PGC1 alpha antibody, Internal used in Western Blot (WB). GTX89046
Anti-PGC1 alpha antibody, Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX89046

Cat. No. GTX89046

Reference 2

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all PGC1 alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-3μg/ml
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

91 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89046-PEP).

Predict Reactivity

Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-DGLFDDSEDESDK, from the internal region of the protein sequence according to NP_037393.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

RRID

AB_10726247

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

PPARG coactivator 1 alpha , LEM6 , PGC-1(alpha) , PGC-1alpha , PGC-1v , PGC1 , PGC1A , PPARGC1

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a transcriptional coactivator that regulates the genes involved in energy metabolism. This protein interacts with PPARgamma, which permits the interaction of this protein with multiple transcription factors. This protein can interact with, and regulate the activities of, cAMP response element binding protein (CREB) and nuclear respiratory factors (NRFs). It provides a direct link between external physiological stimuli and the regulation of mitochondrial biogenesis, and is a major factor that regulates muscle fiber type determination. This protein may be also involved in controlling blood pressure, regulating cellular cholesterol homoeostasis, and the development of obesity. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PGC1 alpha antibody, Internal used in Western Blot (WB). GTX89046

GTX89046 WB Image

WB analysis of human skeletal muscle lysate using GTX89046 PGC1 alpha antibody, Internal.
Dilution : 0.1μg/ml
Loading : 35μg protein in RIPA buffer

Anti-PGC1 alpha antibody, Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX89046

GTX89046 ICC/IF Image

Orthogonal

The data was published in the journal BMC Complement Altern Med in 2019. PMID: 31488120

REFERENCE

Application Reference

Lau WK et al. BMC Complement Altern Med 2019; 19 (1) : 243 Novel discovery of Averrhoa bilimbi ethanolic leaf extract in the stimulation of brown fat differentiation program in combating diet-induced obesity.
Chen CN et al. Am J Physiol Endocrinol Metab. 2015; 308 (11) : 942-9 Late-onset caloric restriction alters skeletal muscle metabolism by modulating pyruvate metabolism.
Application : WB
Reactivity : Rat
Submit a Reference

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399