APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1 - 2 μg/mL |
| 5 μg/mL |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
Mouse Liver Tissue Lysate(GTX27935) , Human Liver Tissue Slide , Human Liver Tissue Lysate
Product Note
At least three isoforms of PPARGC1A are known to exist; this antibody only detects the longest isoform.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
PGC1 alpha antibody was raised against a 19 amino acid peptide near the carboxy terminus of human PGC1 alpha.The immunogen is located within the last 50 amino acids of PGC1 alpha.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
PPARG coactivator 1 alpha , LEM6 , PGC-1(alpha) , PGC-1alpha , PGC-1v , PGC1 , PGC1A , PPARGC1
Cellular Localization
Nucleus
Background
The protein encoded by this gene is a transcriptional coactivator that regulates the genes involved in energy metabolism. This protein interacts with PPARgamma, which permits the interaction of this protein with multiple transcription factors. This protein can interact with, and regulate the activities of, cAMP response element binding protein (CREB) and nuclear respiratory factors (NRFs). It provides a direct link between external physiological stimuli and the regulation of mitochondrial biogenesis, and is a major factor that regulates muscle fiber type determination. This protein may be also involved in controlling blood pressure, regulating cellular cholesterol homoeostasis, and the development of obesity. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX31910 WB Image
WB analysis of human liver tissue lysate using GTX31910 PGC1 alpha antibody.
Working concentration : (A) 1 and (B) 2 μg/ml
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GTX31910 WB Image
WB analysis of mouse liver tissue lysate using GTX31910 PGC1 alpha antibody.
Working concentration : (A) 1 and (B) 2 μg/ml
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GTX31910 IHC-P Image
IHC-P analysis of human liver tissue using GTX31910 PGC1 alpha antibody.
Working concentration : 5 μg/ml
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GTX31910 IHC-P Image
IHC-P analysis of human liver tissue using GTX31910 PGC1 alpha antibody.
Working concentration : 20 μg/ml
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REFERENCE
REVIEW
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