GeneTex
United States (US)

PGC1 alpha antibody

Anti-PGC1 alpha antibody used in Western Blot (WB). GTX31910
Anti-PGC1 alpha antibody used in Western Blot (WB). GTX31910
Anti-PGC1 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX31910
Anti-PGC1 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX31910

Cat No. GTX31910

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, ELISA
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 - 2 μg/mL
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

91 kDa. ( Note )

Positive Control

Mouse Liver Tissue Lysate(GTX27935) , Human Liver Tissue Slide , Human Liver Tissue Lysate

Specificity/Sensitivity

At least three isoforms of PPARGC1A are known to exist; this antibody only detects the longest isoform.
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

PGC1 alpha antibody was raised against a 19 amino acid peptide near the carboxy terminus of human PGC1 alpha.The immunogen is located within the last 50 amino acids of PGC1 alpha.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Pparg Coactivator 1 Alpha,Lem6,Pgc-1(Alpha),Pgc-1Alpha,Pgc-1V,Pgc1,Pgc1A,Ppargc1,Ppargc1A

Background

The protein encoded by this gene is a transcriptional coactivator that regulates the genes involved in energy metabolism. This protein interacts with PPARgamma, which permits the interaction of this protein with multiple transcription factors. This protein can interact with, and regulate the activities of, cAMP response element binding protein (CREB) and nuclear respiratory factors (NRFs). It provides a direct link between external physiological stimuli and the regulation of mitochondrial biogenesis, and is a major factor that regulates muscle fiber type determination. This protein may be also involved in controlling blood pressure, regulating cellular cholesterol homoeostasis, and the development of obesity. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Anti-PGC1 alpha antibody used in Western Blot (WB). GTX31910

GTX31910 WB Image

WB analysis of mouse liver tissue lysate using GTX31910 PGC1 alpha antibody.
Working concentration : (A) 1 and (B) 2 μg/ml

Anti-PGC1 alpha antibody used in Western Blot (WB). GTX31910

GTX31910 WB Image

WB analysis of human liver tissue lysate using GTX31910 PGC1 alpha antibody.
Working concentration : (A) 1 and (B) 2 μg/ml

Anti-PGC1 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX31910

GTX31910 IHC-P Image

IHC-P analysis of human liver tissue using GTX31910 PGC1 alpha antibody.
Working concentration : 20 μg/ml

Anti-PGC1 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX31910

GTX31910 IHC-P Image

IHC-P analysis of human liver tissue using GTX31910 PGC1 alpha antibody.
Working concentration : 5 μg/ml