GeneTex
United States (US)

PGC1 alpha antibody

GTX31921_1589_IHC-P_20180221_18121410_726.jpg
GTX31921_3069_WB_20180221_18121410_490.jpg
GTX31921_1591_IHC-P_20180221_18121410_641.jpg
GTX31921_1590_IHC-P_20180221_18121410_470.jpg
GTX31921_3068_WB_20180221_18121410_725.jpg

Cat No. GTX31921

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, ELISA
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 - 2 μg/mL
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

91 kDa. ( Note )

Positive Control

Human Heart Tissue Lysate(GTX27916)

Specificity/Sensitivity

At least three isoforms are known to exist; this antibody will detect all three isoforms.
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

PGC1 alpha antibody was raised against a 17 amino acid peptide near the amino terminus of human PGC1 alpha.The immunogen is located within amino acids 200 - 250 of PGC1 alpha.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Pparg Coactivator 1 Alpha,Lem6,Pgc-1(Alpha),Pgc-1Alpha,Pgc-1V,Pgc1,Pgc1A,Ppargc1,Ppargc1A

Background

The protein encoded by this gene is a transcriptional coactivator that regulates the genes involved in energy metabolism. This protein interacts with PPARgamma, which permits the interaction of this protein with multiple transcription factors. This protein can interact with, and regulate the activities of, cAMP response element binding protein (CREB) and nuclear respiratory factors (NRFs). It provides a direct link between external physiological stimuli and the regulation of mitochondrial biogenesis, and is a major factor that regulates muscle fiber type determination. This protein may be also involved in controlling blood pressure, regulating cellular cholesterol homoeostasis, and the development of obesity. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX31921_1589_IHC-P_20180221_18121410_726.jpg

GTX31921 IHC-P Image

IHC-P analysis of rat heart tissue using GTX31921 PGC1 alpha antibody.
Working concentration : 5 μg/ml

GTX31921_3069_WB_20180221_18121410_490.jpg

GTX31921 WB Image

WB analysis of human skeletal muscle tissue lysate in (A) the absence and (B) the presence of blocking peptide using GTX31921 PGC1 alpha antibody.
Working concentration : 1 μg/ml

GTX31921_1591_IHC-P_20180221_18121410_641.jpg

GTX31921 IHC-P Image

IHC-P analysis of rat heart tissue using GTX31921 PGC1 alpha antibody.
Working concentration : 20 μg/ml

GTX31921_1590_IHC-P_20180221_18121410_470.jpg

GTX31921 IHC-P Image

IHC-P analysis of rat heart tissue using GTX31921 PGC1 alpha antibody.
Working concentration : 5 μg/ml

GTX31921_3068_WB_20180221_18121410_725.jpg

GTX31921 WB Image

WB analysis of human heart tissue lysate in (A) the absence and (B) the presence of blocking peptide using GTX31921 PGC1 alpha antibody.
Working concentration : 1 μg/ml