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PGC1 alpha antibody

Anti-PGC1 alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31921
Anti-PGC1 alpha antibody used in Western Blot (WB). GTX31921
Anti-PGC1 alpha antibody used in Western Blot (WB). GTX31921
Anti-PGC1 alpha antibody used in Western Blot (WB). GTX31921
Anti-PGC1 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX31921
Anti-PGC1 alpha antibody used in Western Blot (WB). GTX31921

Cat. No. GTX31921

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($419)
See all PGC1 alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 2 μg/mL
ICC/IF 20 μg/mL
IHC-P 1-2 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

91 kDa. ( Note )

Positive Control

Human Heart Tissue Lysate(GTX27916)

Product Note

At least three isoforms are known to exist; this antibody will detect all three isoforms.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

PGC1 alpha antibody was raised against a 17 amino acid peptide near the amino terminus of human PGC1 alpha.The immunogen is located within amino acids 200 - 250 of PGC1 alpha.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887634

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

PPARG coactivator 1 alpha , LEM6 , PGC-1(alpha) , PGC-1alpha , PGC-1v , PGC1 , PGC1A , PPARGC1

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a transcriptional coactivator that regulates the genes involved in energy metabolism. This protein interacts with PPARgamma, which permits the interaction of this protein with multiple transcription factors. This protein can interact with, and regulate the activities of, cAMP response element binding protein (CREB) and nuclear respiratory factors (NRFs). It provides a direct link between external physiological stimuli and the regulation of mitochondrial biogenesis, and is a major factor that regulates muscle fiber type determination. This protein may be also involved in controlling blood pressure, regulating cellular cholesterol homoeostasis, and the development of obesity. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PGC1 alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31921

GTX31921 ICC/IF Image

ICC/IF analysis of 4% PFA-fixed MCF7 cells using GTX31921 PGC1 alpha antibody.
Green : Primary antibody
Blue : DAPI
Dilution : 20 μg/mL

Anti-PGC1 alpha antibody used in Western Blot (WB). GTX31921

GTX31921 WB Image

WB analysis of various samples using GTX31921 PGC1 alpha antibody.
Lane 1 : Daudi cell lysate
Lane 2 : K562 cell lysate
Loading : 15 μg
Dilution : 2 μg/mL

Anti-PGC1 alpha antibody used in Western Blot (WB). GTX31921

GTX31921 WB Image

WB analysis of mouse skin tissue lysate using GTX31921 PGC1 alpha antibody.
Loading : 15 μg
Dilution : 2 μg/mL

Anti-PGC1 alpha antibody used in Western Blot (WB). GTX31921

GTX31921 WB Image

WB analysis of rat kidney tissue lysate using GTX31921 PGC1 alpha antibody.
Loading : 15 μg
Dilution : 2 μg/mL

Anti-PGC1 alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX31921

GTX31921 IHC-P Image

IHC-P analysis of human colon carcinoma tissue using GTX31921 PGC1 alpha antibody.
Antigen retrieval: Heat mediated antigen retrieval in citrate buffer (pH 6).
Dilution : 1 μg/mL

Anti-PGC1 alpha antibody used in Western Blot (WB). GTX31921

GTX31921 WB Image

WB analysis of human lung tissue lysate using GTX31921 PGC1 alpha antibody.
Loading : 15 μg
Dilution : 2 μg/mL

REFERENCE

Application Reference

Yang YL et al. Int J Mol Sci 2020; 21 (18) miR-29a Modulates GSK3β/SIRT1-Linked Mitochondrial Proteostatic Stress to Ameliorate Mouse Non-Alcoholic Steatohepatitis.
Colitti M et al. Tissue Cell 2020; 66 : 101388 Brain-derived neurotrophic factor modulates mitochondrial dynamics and thermogenic phenotype on 3T3-L1 adipocytes.
Application : WB
Reactivity : Mouse
Submit a Reference

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 419