GeneTex
United States (US)

PGD antibody [N1N3]

GTX101703_39946_20150410_IFA_19011506_262.jpg
GTX101703_WB_19011506_339.jpg
GTX101703_39946_20151015_WB_M_heart_19011506_106.jpg
GTX101703_39946_IFA_19011506_137.jpg
GTX101703_IHC_19011506_275.jpg
GTX101703_39946_20151029_WB_M_lung_19011506_846.jpg

Cat No. GTX101703

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

Positive Control

293T , Molt-4 , Raji , Mouse heart , Mouse lung

Predict Reactivity

Rat, Zebrafish, Sheep, Bovine, Chicken, Xenopus laevis, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PGD. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Phosphogluconate Dehydrogenase,6Pgd,Pgd

Background

6-phosphogluconate dehydrogenase is the second dehydrogenase in the pentose phosphate shunt. Deficiency of this enzyme is generally asymptomatic, and the inheritance of this disorder is autosomal dominant. Hemolysis results from combined deficiency of 6-phosphogluconate dehydrogenase and 6-phosphogluconolactonase suggesting a synergism of the two enzymopathies. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101703_39946_20150410_IFA_19011506_262.jpg

GTX101703 ICC/IF Image

PGD antibody [N1N3] detects PGD protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PGD protein stained by PGD antibody [N1N3] (GTX101703) diluted at 1:1000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.

GTX101703_WB_19011506_339.jpg

GTX101703 WB Image

Sample(30 µg whole cell lysate)
A:293T
B:MOLT4 (GTX27912)
C:Raji (GTX27908)
10% SDS PAGE
GTX101703 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101703_39946_20151015_WB_M_heart_19011506_106.jpg

GTX101703 WB Image

PGD antibody [N1N3] detects PGD protein by western blot analysis. Mouse tissue extracts (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with PGD antibody [N1N3] (GTX101703) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101703_39946_IFA_19011506_137.jpg

GTX101703 ICC/IF Image

PGD antibody [N1N3] detects PGD protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: PGD protein stained by PGD antibody [N1N3] (GTX101703) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX101703_IHC_19011506_275.jpg

GTX101703 IHC-P Image

PGD antibody [N1N3] detects PGD protein at cytoplasm on A549 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded A549 xenograft.
PGD antibody [N1N3] (GTX101703) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101703_39946_20151029_WB_M_lung_19011506_846.jpg

GTX101703 WB Image

PGD antibody [N1N3] detects PGD protein by western blot analysis. Mouse tissue extracts (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with PGD antibody [N1N3] (GTX101703) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169