GeneTex
United States (US)

PGD antibody

GTX101704_40632_IFA_19011506_699.jpg
GTX101704_40632_WB_19011506_181.jpg
GTX101704_40632_20151029_WB_M_lung_19011506_775.jpg
GTX101704_40632_20141128_IHC_19011506_634.jpg
GTX101704_40632_20151015_WB_M_heart_19011506_300.jpg

Cat No. GTX101704

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

Positive Control

A549 , Mouse heart , Mouse lung

Predict Reactivity

Rat, Zebrafish, Sheep, Bovine, Chicken, Xenopus laevis, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PGD. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

6PGD antibody, PGD antibody, "6-phosphogluconate dehydrogenase, decarboxylating antibody", phosphogluconate dehydrogenase antibody

Background

6-phosphogluconate dehydrogenase is the second dehydrogenase in the pentose phosphate shunt. Deficiency of this enzyme is generally asymptomatic, and the inheritance of this disorder is autosomal dominant. Hemolysis results from combined deficiency of 6-phosphogluconate dehydrogenase and 6-phosphogluconolactonase suggesting a synergism of the two enzymopathies. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101704_40632_IFA_19011506_699.jpg

GTX101704 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A549, using PGD(GTX101704) antibody at 1:500 dilution.

GTX101704_40632_WB_19011506_181.jpg

GTX101704 WB Image

Sample (30 ug of whole cell lysate)
A: A549
10% SDS PAGE
GTX101704 diluted at 1:1000

GTX101704_40632_20151029_WB_M_lung_19011506_775.jpg

GTX101704 WB Image

PGD antibody detects PGD protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PGD antibody (GTX101704) diluted at 1:500.

GTX101704_40632_20141128_IHC_19011506_634.jpg

GTX101704 IHC-P Image

PGD antibody detects PGD protein at cytoplasm on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
PGD antibody (GTX101704) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101704_40632_20151015_WB_M_heart_19011506_300.jpg

GTX101704 WB Image

PGD antibody detects PGD protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PGD antibody (GTX101704) diluted at 1:1000.

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169