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PGD antibody

Anti-PGD antibody used in Western Blot (WB). GTX101704
Anti-PGD antibody used in Western Blot (WB). GTX101704
Anti-PGD antibody used in Western Blot (WB). GTX101704
Anti-PGD antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101704
Anti-PGD antibody used in IHC (Paraffin sections) (IHC-P). GTX101704

Cat. No. GTX101704

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all PGD products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

Positive Control

A549 , mouse heart , mouse lung

Predict Reactivity

Rat, Zebrafish, Sheep, Bovine, Chicken, Xenopus laevis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PGD. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11175654

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

phosphogluconate dehydrogenase , 6PGD

Background

6-phosphogluconate dehydrogenase is the second dehydrogenase in the pentose phosphate shunt. Deficiency of this enzyme is generally asymptomatic, and the inheritance of this disorder is autosomal dominant. Hemolysis results from combined deficiency of 6-phosphogluconate dehydrogenase and 6-phosphogluconolactonase suggesting a synergism of the two enzymopathies. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PGD antibody used in Western Blot (WB). GTX101704

GTX101704 WB Image

PGD antibody detects PGD protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PGD antibody (GTX101704) diluted at 1:500.

Anti-PGD antibody used in Western Blot (WB). GTX101704

GTX101704 WB Image

Sample (30 ug of whole cell lysate)
A: A549
10% SDS PAGE
GTX101704 diluted at 1:1000

Anti-PGD antibody used in Western Blot (WB). GTX101704

GTX101704 WB Image

PGD antibody detects PGD protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PGD antibody (GTX101704) diluted at 1:1000.

Anti-PGD antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101704

GTX101704 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A549, using PGD(GTX101704) antibody at 1:500 dilution.

Anti-PGD antibody used in IHC (Paraffin sections) (IHC-P). GTX101704

GTX101704 IHC-P Image

PGD antibody detects PGD protein at cytoplasm on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
PGD antibody (GTX101704) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169