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PGK1 + PGK2 antibody [HL1775]

Recombinant antibody
Anti-PGK1 + PGK2 antibody [HL1775] used in Western Blot (WB). GTX637427
Anti-PGK1 + PGK2 antibody [HL1775] used in Western Blot (WB). GTX637427
Anti-PGK1 + PGK2 antibody [HL1775] used in IHC (Paraffin sections) (IHC-P). GTX637427
Anti-PGK1 + PGK2 antibody [HL1775] used in IHC (Paraffin sections) (IHC-P). GTX637427
Anti-PGK1 + PGK2 antibody [HL1775] used in Western Blot (WB). GTX637427

Cat. No. GTX637427

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1775

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all PGK1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein of human PGK1.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

phosphoglycerate kinase 1 , HEL-S-68p , MIG10 , PGKA

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. Additionally, this protein is secreted by tumor cells where it participates in angiogenesis by functioning to reduce disulfide bonds in the serine protease, plasmin, which consequently leads to the release of the tumor blood vessel inhibitor angiostatin. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Deficiency of the enzyme is associated with a wide range of clinical phenotypes hemolytic anemia and neurological impairment. Pseudogenes of this gene have been defined on chromosomes 19, 21 and the X chromosome. [provided by RefSeq, Jan 2014]

Database

Research Area

DATA IMAGES

Anti-PGK1 + PGK2 antibody [HL1775] used in Western Blot (WB). GTX637427

GTX637427 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PGK1 + PGK2 antibody [HL1775] (GTX637427) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PGK1 + PGK2 antibody [HL1775] used in Western Blot (WB). GTX637427

GTX637427 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PGK1 + PGK2 antibody [HL1775] (GTX637427) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PGK1 + PGK2 antibody [HL1775] used in IHC (Paraffin sections) (IHC-P). GTX637427

GTX637427 IHC-P Image

PGK1 + PGK2 antibody [HL1775] detects PGK1 + PGK2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
PGK1 stained by PGK1 + PGK2 antibody [HL1775] (GTX637427) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PGK1 + PGK2 antibody [HL1775] used in IHC (Paraffin sections) (IHC-P). GTX637427

GTX637427 IHC-P Image

PGK1 + PGK2 antibody [HL1775] detects PGK1 + PGK2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
PGK1 stained by PGK1 + PGK2 antibody [HL1775] (GTX637427) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PGK1 + PGK2 antibody [HL1775] used in Western Blot (WB). GTX637427

GTX637427 WB Image

Whole zebrafish extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PGK1 + PGK2 antibody [HL1775] (GTX637427) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations