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PGK1 antibody [AT2F4]

Anti-PGK1 antibody [AT2F4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57637
Anti-PGK1 antibody [AT2F4] used in Western Blot (WB). GTX57637

Cat. No. GTX57637

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 AT2F4

Isotype

 IgG1

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399)
See all PGK1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The clone AT2F4 is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human PGK3 protein.

Purification

Protein A Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

phosphoglycerate kinase 1 , HELS68p , MIG10 , PGK1 , PGKA

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. Additionally, this protein is secreted by tumor cells where it participates in angiogenesis by functioning to reduce disulfide bonds in the serine protease, plasmin, which consequently leads to the release of the tumor blood vessel inhibitor angiostatin. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Deficiency of the enzyme is associated with a wide range of clinical phenotypes hemolytic anemia and neurological impairment. Pseudogenes of this gene have been defined on chromosomes 19, 21 and the X chromosome. [provided by RefSeq, Jan 2014]

Database

Research Area

DATA IMAGES

Anti-PGK1 antibody [AT2F4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57637

GTX57637 ICC/IF Image

ICC/IF analysis of U87-MG cells using GTX57637 PGK1 antibody.
Blue: DAPI
Green: Primary antibody
Dilution: 1:100

Anti-PGK1 antibody [AT2F4] used in Western Blot (WB). GTX57637

GTX57637 WB Image

WB analysis of various samples using GTX57637 PGK1 antibody.
Lane 1 : HeLa whole cell lysate
Lane 2 : LNCap whole cell lysate
Lane 3 : A549 whole cell lysate
Lane 4 : HepG2 whole cell lysate
Lane 5 : 293T whole cell lysate
Lane 6 : K562 whole cell lysate
Lane 7 : Jurkat whole cell lysate
Loading : 40 μg
Dilution : 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399