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PGK1 antibody [GT21612]

Overexpression
Anti-PGK1 antibody [GT21612] used in Western Blot (WB). GTX634447
Anti-PGK1 antibody [GT21612] used in IHC (Paraffin sections) (IHC-P). GTX634447
Anti-PGK1 antibody [GT21612] used in Western Blot (WB). GTX634447

Cat. No. GTX634447

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT21612

Isotype

 IgG2a

Application

 WB, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all PGK1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

HeLa , PGK1-transfected 293T

Product Note

Based on sequence homology, this antibody may cross react with PGK2 protein.

Predict Reactivity

Mouse, Zebrafish, Sheep, Bovine, Pig, Xenopus laevis, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PGK1. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888451

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

phosphoglycerate kinase 1 , HEL-S-68p , MIG10 , PGKA

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. This gene lies on the X-chromosome, while a related pseudogene also has been found on the X-chromosome and another on chromosome 19. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PGK1 antibody [GT21612] used in Western Blot (WB). GTX634447

GTX634447 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PGK1 antibody [GT21612] (GTX634447) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-PGK1 antibody [GT21612] used in IHC (Paraffin sections) (IHC-P). GTX634447

GTX634447 IHC-P Image

PGK1 antibody [GT21612] detects PGK1 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
PGK1 stained by PGK1 antibody [GT21612] (GTX634447) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PGK1 antibody [GT21612] used in Western Blot (WB). GTX634447

GTX634447 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PGK1 antibody [GT21612] (GTX634447) diluted at 1:3000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations