GeneTex
  • Country / Location Selection

United States (US)

PGK1 antibody [GT21612]

Anti-PGK1 antibody [GT21612] used in Western Blot (WB). GTX634447
Anti-PGK1 antibody [GT21612] used in Western Blot (WB). GTX634447
Anti-PGK1 antibody [GT21612] used in IHC (Paraffin sections) (IHC-P). GTX634447

Cat No. GTX634447

Host

Mouse

Clonality

Monoclonal

Clone Name

GT21612

Isotype

IgG2a

Application

WB, IHC-P

Reactivity

Human, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Mouse, Zebrafish, Sheep, Bovine, Chicken, Pig, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PGK1. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888451

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

phosphoglycerate kinase 1 , HEL-S-68p , MIG10 , PGKA

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. This gene lies on the X-chromosome, while a related pseudogene also has been found on the X-chromosome and another on chromosome 19. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PGK1 antibody [GT21612] used in Western Blot (WB). GTX634447

GTX634447 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with PGK1 antibody [GT21612] (GTX634447) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-PGK1 antibody [GT21612] used in Western Blot (WB). GTX634447

GTX634447 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PGK1 antibody [GT21612] (GTX634447) diluted at 1:3000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-PGK1 antibody [GT21612] used in IHC (Paraffin sections) (IHC-P). GTX634447

GTX634447 IHC-P Image

PGK1 antibody [GT21612] detects PGK1 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
PGK1 stained by PGK1 antibody [GT21612] (GTX634447) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

There are currently no references for PGK1 antibody [GT21612] (GTX634447). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for PGK1 antibody [GT21612] (GTX634447). Be the first to share your experience with this product.
SDS
PBS.pdf
Package List Price ($)
$ 329
$ 169
Custom Conjugations