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PGK1 antibody [N1C1]

KO/KD
Anti-PGK1 antibody [N1C1] used in Western Blot (WB). GTX101405
Anti-PGK1 antibody [N1C1] used in Western Blot (WB). GTX101405
Anti-PGK1 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX101405
Anti-PGK1 antibody [N1C1] used in Western Blot (WB). GTX101405
Anti-PGK1 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX101405

Cat. No. GTX101405

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all PGK1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

Mouse brain , 293T , A431 , H1299

Predict Reactivity

Zebrafish, Sheep, Bovine, Pig, Xenopus laevis, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PGK1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037668

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

phosphoglycerate kinase 1 , HEL-S-68p , MIG10 , PGKA

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. This gene lies on the X-chromosome, while a related pseudogene also has been found on the X-chromosome and another on chromosome 19. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PGK1 antibody [N1C1] used in Western Blot (WB). GTX101405

GTX101405 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PGK1 antibody [N1C1] (GTX101405) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PGK1 antibody [N1C1] used in Western Blot (WB). GTX101405

GTX101405 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: A431 (GTX27909)
C: H1299
10% SDS PAGE
GTX101405 diluted at 1:1000

Anti-PGK1 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX101405

GTX101405 IHC-P Image

PGK1 antibody [N1C1] detects PGK1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
PGK1 stained by PGK1 antibody [N1C1] (GTX101405) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PGK1 antibody [N1C1] used in Western Blot (WB). GTX101405

GTX101405 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX101405 diluted at 1:1000

Anti-PGK1 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX101405

GTX101405 IHC-P Image

PGK1 antibody [N1C1] detects PGK1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
PGK1 stained by PGK1 antibody [N1C1] (GTX101405) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Lin LH et al. Electrophoresis 2014; Biomarker discovery for neuroendocrine cervical cancer.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169