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PGK1 antibody [N1C1]

Anti-PGK1 antibody [N1C1] used in Western Blot (WB). GTX101405
Anti-PGK1 antibody [N1C1] used in Western Blot (WB). GTX101405
Anti-PGK1 antibody [N1C1] used in Western Blot (WB). GTX101405
Anti-PGK1 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX101405
Anti-PGK1 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX101405

Cat No. GTX101405

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , mouse brain , PGK1 shRNA-transfected 293T

Predict Reactivity

Zebrafish, Sheep, Bovine, Chicken, Pig, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PGK1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037668

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

phosphoglycerate kinase 1 , HEL-S-68p , MIG10 , PGKA

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. This gene lies on the X-chromosome, while a related pseudogene also has been found on the X-chromosome and another on chromosome 19. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PGK1 antibody [N1C1] used in Western Blot (WB). GTX101405

GTX101405 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PGK1 antibody [N1C1] (GTX101405) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PGK1 antibody [N1C1] used in Western Blot (WB). GTX101405

GTX101405 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: A431 (GTX27909)
C: H1299
10% SDS PAGE
GTX101405 diluted at 1:1000

Anti-PGK1 antibody [N1C1] used in Western Blot (WB). GTX101405

GTX101405 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX101405 diluted at 1:1000

Anti-PGK1 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX101405

GTX101405 IHC-P Image

PGK1 antibody [N1C1] detects PGK1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
PGK1 stained by PGK1 antibody [N1C1] (GTX101405) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PGK1 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX101405

GTX101405 IHC-P Image

PGK1 antibody [N1C1] detects PGK1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
PGK1 stained by PGK1 antibody [N1C1] (GTX101405) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169