GeneTex
United States (US)

PGK1 antibody

KO/KD Orthogonal Overexpression
GTX107614_41878_20141107_WB_19011506_856.jpg
GTX107614_41878_20181221_WB_shRNA_watermark_19011519_715.jpg
GTX107614_41878_20181026_WB_B_19011519_431.jpg
GTX107614_40009_WB_R_liver_19011506_202.jpg
GTX107614_IFA_19011506_560.jpg
GTX107614_40009_20150410_IFA_19011506_901.jpg
GTX107614_40009_WB_Hypoxia_19011506_374.jpg
GTX107614_40009_WB_M_brain_19011506_124.jpg
GTX107614_40009_IHC_19011506_425.jpg

Cat No. GTX107614

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, ELISA, PLA
Reactivity Human, Mouse, Rat, Yeast
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:20-1:1000
IHC-P 1:100-1:1000
ELISA Assay dependent
PLA Assay dependent
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

293T , PGK1 shRNA-transfected 293T , A431 , HeLa , HepG2 , mouse brain , Rat liver , *Yeast wild type 303

Predict Reactivity

Zebrafish, Sheep, Bovine, Chicken, Pig, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.048mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PGK1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

MGC117307 antibody, MGC142128 antibody, MGC8947 antibody, MIG10 antibody, PGKA antibody, PGK1 antibody, phosphoglycerate kinase 1 antibody, cell migration-inducing gene 10 protein antibody, PRP 2 antibody, primer recognition protein 2 antibody

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. This gene lies on the X-chromosome, while a related pseudogene also has been found on the X-chromosome and another on chromosome 19. [provided by RefSeq]
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Database

Research Area

DATA IMAGES
GTX107614_41878_20141107_WB_19011506_856.jpg

GTX107614 WB Image

PGK1 antibody detects PGK1 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with PGK1 antibody (GTX107614) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX107614_41878_20181221_WB_shRNA_watermark_19011519_715.jpg

GTX107614 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with PGK1 antibody (GTX107614) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX107614_41878_20181026_WB_B_19011519_431.jpg

GTX107614 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with PGK1 antibody (GTX107614) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX107614_40009_WB_R_liver_19011506_202.jpg

GTX107614 WB Image

PGK1 antibody detects PGK1 protein by western blot analysis.
A. 50 µg Rat liver lysate/extract
10% SDS-PAGE
PGK1 antibody (GTX107614) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX107614_IFA_19011506_560.jpg

GTX107614 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using PGK1(GTX107614) antibody at 1:50 dilution.

GTX107614_40009_20150410_IFA_19011506_901.jpg

GTX107614 ICC/IF Image

PGK1 antibody detects PGK1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PGK1 protein stained by PGK1 antibody (GTX107614) diluted at 1:20.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000.
Blue: Hoechst 33342 staining.

GTX107614_40009_WB_Hypoxia_19011506_374.jpg

GTX107614 WB Image

Orthogonal

PGK1 antibody detects PGK1 protein in 1% O2-treated samples by western blot analysis.
Upper panel: PGK1 antibody (GTX107614)
Lower panel: beta Actin antibody (GTX109639)
A. 30 µg HeLa whole cell lysate/extract (untreated)
B. 30 µg HeLa whole cell lysate/extract (1% O2 treatment for 24hr)
10% SDS PAGE 
PGK1 antibody (GTX107614) dilution: 1:1000
beta Actin antibody (GTX109639) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX107614_40009_WB_M_brain_19011506_124.jpg

GTX107614 WB Image

Sample (50 µg of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX107614 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX107614_40009_IHC_19011506_425.jpg

GTX107614 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using PGK1 antibody(GTX107614) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Reference
REVIEW

PGK1 antibody Cat No. GTX107614

Rating ( Average 5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 5 based on 2 users reviews)
Date : Anonymous submitted on 29-Aug-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : OEC-M1 cells
Amount used : 30 μg
Blocking : 5% non-fat milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 25°C, 1Hr
Date : Anonymous submitted on 03-May-2012
Score :
Application Tested : WB
Sample Species : Yeast
Sample : Yeast wild type 303
Amount used : 10μg
Blocking : 2% milk in TBST, 25°C, 2Hr
Primary Antibody : 1:500, 25°C, 2Hr
Figure Note : Amino acid 163~177 cross react to yeast
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf