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PGK1 antibody

Anti-PGK1 antibody used in Western Blot (WB). GTX107614
Anti-PGK1 antibody used in Western Blot (WB). GTX107614
Anti-PGK1 antibody used in Western Blot (WB). GTX107614
Anti-PGK1 antibody used in Western Blot (WB). GTX107614
Anti-PGK1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107614
Anti-PGK1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107614
Anti-PGK1 antibody used in Western Blot (WB). GTX107614
Anti-PGK1 antibody used in Western Blot (WB). GTX107614
Anti-PGK1 antibody used in IHC (Paraffin sections) (IHC-P). GTX107614

Cat No. GTX107614

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP, ELISA, PLA

Reactivity

Human, Mouse, Rat, Yeast
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:20-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
ELISA Assay dependent
PLA Assay dependent
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

293T , PGK1 shRNA-transfected 293T , A431 , HeLa , HepG2 , mouse brain , Rat liver , *Yeast wild type 303

Predict Reactivity

Zebrafish, Sheep, Bovine, Chicken, Pig, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.048 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PGK1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037666

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

phosphoglycerate kinase 1 , HEL-S-68p , MIG10 , PGKA

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. This gene lies on the X-chromosome, while a related pseudogene also has been found on the X-chromosome and another on chromosome 19. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PGK1 antibody used in Western Blot (WB). GTX107614

GTX107614 WB Image

PGK1 antibody detects PGK1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PGK1 antibody (GTX107614) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PGK1 antibody used in Western Blot (WB). GTX107614

GTX107614 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PGK1 antibody (GTX107614) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PGK1 antibody used in Western Blot (WB). GTX107614

GTX107614 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PGK1 antibody (GTX107614) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PGK1 antibody used in Western Blot (WB). GTX107614

GTX107614 WB Image

PGK1 antibody detects PGK1 protein by western blot analysis.
A. 50 μg Rat liver lysate/extract
10% SDS-PAGE
PGK1 antibody (GTX107614) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PGK1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107614

GTX107614 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using PGK1(GTX107614) antibody at 1:50 dilution.

Anti-PGK1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107614

GTX107614 ICC/IF Image

PGK1 antibody detects PGK1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PGK1 protein stained by PGK1 antibody (GTX107614) diluted at 1:20.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000.
Blue: Hoechst 33342 staining.

Anti-PGK1 antibody used in Western Blot (WB). GTX107614

GTX107614 WB Image

PGK1 antibody detects PGK1 protein in 1% O2-treated samples by western blot analysis.
Upper panel: PGK1 antibody (GTX107614)
Lower panel: beta Actin antibody (GTX109639)
A. 30 μg HeLa whole cell lysate/extract (untreated)
B. 30 μg HeLa whole cell lysate/extract (1% O2 treatment for 24hr)
10% SDS PAGE 
PGK1 antibody (GTX107614) dilution: 1:1000
beta Actin antibody (GTX109639) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PGK1 antibody used in Western Blot (WB). GTX107614

GTX107614 WB Image

Sample (50 μg of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX107614 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PGK1 antibody used in IHC (Paraffin sections) (IHC-P). GTX107614

GTX107614 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using PGK1 antibody(GTX107614) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

REVIEW

PGK1 antibody Cat No. GTX107614

Rating ( Average 5 based on 3 users reviews)
Application
Western Blot(WB) ( Average 5 based on 3 users reviews)
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal BMC Genomics in 2015.PMID: 25652794
Date : Anonymous submitted on 29-Aug-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : OEC-M1 cells
Amount used : 30 μg
Blocking : 5% non-fat milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 25°C, 1Hr
Date : Anonymous submitted on 03-May-2012
Score :
Application Tested : WB
Sample Species : Yeast
Sample : Yeast wild type 303
Amount used : 10μg
Blocking : 2% milk in TBST, 25°C, 2Hr
Primary Antibody : 1:500, 25°C, 2Hr
Figure Note : Amino acid 163~177 cross react to yeast
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf