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PGK1 antibody

KO/KD Orthogonal Overexpression
Anti-PGK1 antibody used in Western Blot (WB). GTX107614
Anti-PGK1 antibody used in Western Blot (WB). GTX107614
Anti-PGK1 antibody used in Western Blot (WB). GTX107614
Anti-PGK1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107614
Anti-PGK1 antibody used in Western Blot (WB). GTX107614
Anti-PGK1 antibody used in IHC (Paraffin sections) (IHC-P). GTX107614
Anti-PGK1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107614
Anti-PGK1 antibody used in Western Blot (WB). GTX107614
Anti-PGK1 antibody used in Western Blot (WB). GTX107614
Anti-PGK1 antibody used in Western Blot (WB). GTX107614

Cat. No. GTX107614

Reference 14
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP, ELISA, PLA

Reactivity

 Human, Mouse, Rat, Yeast
Package
100 μl ($399),
25 μl ($169)
See all PGK1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:20-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
ELISA Assay dependent
PLA Assay dependent
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

HeLa (1% O2 treatment for 24hr) , Mouse brain , Rat liver , 293T , A431 , HeLa , HepG2 , PGK1-transfected 293T

Predict Reactivity

Zebrafish, Sheep, Bovine, Pig, Xenopus laevis, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.05 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PGK1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037666

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

phosphoglycerate kinase 1 , HEL-S-68p , MIG10 , PGKA

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. This gene lies on the X-chromosome, while a related pseudogene also has been found on the X-chromosome and another on chromosome 19. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PGK1 antibody used in Western Blot (WB). GTX107614

GTX107614 WB Image

PGK1 antibody detects PGK1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PGK1 antibody (GTX107614) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PGK1 antibody used in Western Blot (WB). GTX107614

GTX107614 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PGK1 antibody (GTX107614) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PGK1 antibody used in Western Blot (WB). GTX107614

GTX107614 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PGK1 antibody (GTX107614) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PGK1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107614

GTX107614 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using PGK1(GTX107614) antibody at 1:50 dilution.

Anti-PGK1 antibody used in Western Blot (WB). GTX107614

GTX107614 WB Image

Sample (50 μg of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX107614 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PGK1 antibody used in IHC (Paraffin sections) (IHC-P). GTX107614

GTX107614 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using PGK1 antibody(GTX107614) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PGK1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107614

GTX107614 ICC/IF Image

PGK1 antibody detects PGK1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PGK1 protein stained by PGK1 antibody (GTX107614) diluted at 1:20.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000.
Blue: Hoechst 33342 staining.
Anti-PGK1 antibody used in Western Blot (WB). GTX107614

GTX107614 WB Image

PGK1 antibody detects PGK1 protein by western blot analysis.
A. 50 μg Rat liver lysate/extract
10% SDS-PAGE
PGK1 antibody (GTX107614) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PGK1 antibody used in Western Blot (WB). GTX107614

GTX107614 WB Image

Orthogonal

PGK1 antibody detects PGK1 protein in 1% O2-treated samples by western blot analysis.
Upper panel: PGK1 antibody (GTX107614)
Lower panel: beta Actin antibody (GTX109639)
A. 30 μg HeLa whole cell lysate/extract (untreated)
B. 30 μg HeLa whole cell lysate/extract (1% O2 treatment for 24hr)
10% SDS PAGE 
PGK1 antibody (GTX107614) dilution: 1:1000
beta Actin antibody (GTX109639) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PGK1 antibody used in Western Blot (WB). GTX107614

GTX107614 WB Image

The data was published in the journal BMC Genomics in 2015. PMID: 25652794

REFERENCE

Application Reference

Luo Y et al. Exp Anim 2023; 72 (4) : 475-489 Phosphoglycerate kinase (PGK) 1 succinylation modulates epileptic seizures and the blood-brain barrier.
Ogawa T et al. Physiol Rep 2023; 11 (4) : e15608 Downregulation of extramitochondrial BCKDH and its uncoupling from AMP deaminase in type 2 diabetic OLETF rat hearts.
Application : WB
Reactivity : Rat
Kerrie-Ann McMahon et al. Elife 2021; 10 Cavin3 released from caveolae interacts with BRCA1 to regulate the cellular stress response.
Application : WB
Reactivity : Human
Chang YC et al. Cell Death Discov 2021; 7 (1) : 135 Metabolic protein phosphoglycerate kinase 1 confers lung cancer migration by directly binding HIV Tat specific factor 1.
Application : IP
Reactivity : Human
Somatilaka BN et al. Dev Cell 2020; 54 (6) : 710-726.e8 Ankmy2 Prevents Smoothened-Independent Hyperactivation of the Hedgehog Pathway via Cilia-Regulated Adenylyl Cyclase Signaling.
Application : WB
Reactivity : Mouse
Show More +
Submit a Reference

REVIEW

PGK1 antibody Cat. No. GTX107614

Rating
( Average 5 based on 2 users reviews)
Application
Western Blot(WB)
( Average 5 based on 2 users reviews)
Anti-PGK1 antibody used in Western Blot (WB). GTX107614
Date : Anonymous submitted on 29-Aug-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : OEC-M1 cells
Amount used : 30 μg
Blocking : 5% non-fat milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 25°C, 1Hr
Anti-PGK1 antibody used in Western Blot (WB). GTX107614
Date : Anonymous submitted on 03-May-2012
Score :
Application Tested : WB
Sample Species : Yeast
Sample : Yeast wild type 303
Amount used : 10μg
Blocking : 2% milk in TBST, 25°C, 2Hr
Primary Antibody : 1:500, 25°C, 2Hr
Figure Note : Amino acid 163~177 cross react to yeast
Submit a Review
Application Reference
Luo Y et al. Exp Anim 2023; 72 (4):475-489 Phosphoglycerate kinase (PGK) 1 succinylation modulates epileptic seizures and the blood-brain barrier.
Ogawa T et al. Physiol Rep 2023; 11 (4):e15608 Downregulation of extramitochondrial BCKDH and its uncoupling from AMP deaminase in type 2 diabetic OLETF rat hearts.
Application : WB
Reactivity : Rat
Kerrie-Ann McMahon et al. Elife 2021; 10 Cavin3 released from caveolae interacts with BRCA1 to regulate the cellular stress response.
Application : WB
Reactivity : Human
Chang YC et al. Cell Death Discov 2021; 7 (1):135 Metabolic protein phosphoglycerate kinase 1 confers lung cancer migration by directly binding HIV Tat specific factor 1.
Application : IP
Reactivity : Human
Somatilaka BN et al. Dev Cell 2020; 54 (6):710-726.e8 Ankmy2 Prevents Smoothened-Independent Hyperactivation of the Hedgehog Pathway via Cilia-Regulated Adenylyl Cyclase Signaling.
Application : WB
Reactivity : Mouse
Nan H et al. Nat Commun 2019; 10 (1):359 A viral expression factor behaves as a prion.
Yeh YH et al. J Exp Clin Cancer Res 2018; 37 (1):70 inflammatory * activates hif-1α-mediated epithelial-to-mesenchymal transition via pi3k/akt/mtor pathway.
Application : WB
Reactivity : Human
Chang SJ et al. Arch Biochem Biophys 2018; 647:10-32 Proteomic investigating the cooperative lethal effect of EGFR and MDM2 inhibitors on ovarian carcinoma.
Application : WB
Reactivity : Human
Silva LS et al. EMBO Rep 2017; (Epub) Branched-chain ketoacids secreted by glioblastoma cells via MCT1 modulate macrophage phenotype.
Application : PLA
Reactivity : Human
Lin LL et al. BMC Genomics 2015; 16:41 Integrating transcriptomics and proteomics to show that tanshinone IIA suppresses cell growth by blocking glucose metabolism in gastric cancer cells.
Application : WB
Reactivity : Human
Drusenheimer N et al. PLoS Genet 2015; 11 (12):e1005749 The Mammalian Orthologs of Drosophila Lgd, CC2D1A and CC2D1B, Function in the Endocytic Pathway, but Their Individual Loss of Function Does Not Affect Notch Signalling.
Lee SO et al. Mol Cancer Res 2014; 12 (4):527-538 The orphan nuclear receptor NR4A1 (Nur77) regulates oxidative and endoplasmic reticulum stress in pancreatic cancer cells.
Application : WB
Reactivity : Human
Wu YH et al. PLoS One 2013; 8 (8):e70642 Quantitative proteomic analysis of human lung tumor xenografts treated with the ectopic ATP synthase inhibitor citreoviridin.
Application : WB
Reactivity : Human
Chen JY et al. J Nutr Biochem 2013; 24 (11):1889-910 High glucose-induced proteome alterations in hepatocytes and its possible relevance to diabetic liver disease.
Application : ELISA
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169