GeneTex
United States (US)

PGK1 antibody

GTX32781_20180530_WB_18121410_390.jpg
GTX32781_20180530_ICCIF_18121410_341.jpg

Cat No. GTX32781

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
Not tested in other applications.

Calculated MW

45 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-417 of human PGK1 (NP_000282.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Phosphoglycerate Kinase 1,Hel-S-68P,Mig10,Pgka,Pgk1

Background

The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. Additionally, this protein is secreted by tumor cells where it participates in angiogenesis by functioning to reduce disulfide bonds in the serine protease, plasmin, which consequently leads to the release of the tumor blood vessel inhibitor angiostatin. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Deficiency of the enzyme is associated with a wide range of clinical phenotypes hemolytic anemia and neurological impairment. Pseudogenes of this gene have been defined on chromosomes 19, 21 and the X chromosome. [provided by RefSeq, Jan 2014]

Database

Research Area

DATA IMAGES
GTX32781_20180530_WB_18121410_390.jpg

GTX32781 WB Image

WB analysis of various samples using GTX32781 PGK1 antibody.
Dilution : 1:1000
Loading : 25μg per lane

GTX32781_20180530_ICCIF_18121410_341.jpg

GTX32781 ICC/IF Image

ICC/IF analysis of HeLa cell using GTX32781 PGK1 antibody.
Blue : DAPI

Package List Price ($)
$ 329