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PGK2 antibody [N3C3]

Anti-PGK2 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX107837
Anti-PGK2 antibody [N3C3] used in Western Blot (WB). GTX107837

Cat. No. GTX107837

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all PGK2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

293T , HeLaS3

Predict Reactivity

Mouse, Rat, Bovine, Chicken, Pig, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.19 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PGK2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951207

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

phosphoglycerate kinase 2 , HEL-S-272 , PGKB , PGKPS , dJ417L20.2

Cellular Localization

Cytoplasm

Background

The PGK2 gene encodes a testis-specific form of phosphoglycerate kinase (EC 2.7.2.3), which catalyzes the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate during glycolysis, generating one molecule of ATP. See also PGK1 (MIM 311800), which is ubiquitously expressed in all somatic tissues and maps to chromosome Xq13.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-PGK2 antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX107837

GTX107837 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human mixed ovarian cancer, using PGK2(GTX107837) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PGK2 antibody [N3C3] used in Western Blot (WB). GTX107837

GTX107837 WB Image

Sample(30 μg of whole cell lysate)
A:293T
B:HeLa S3(GTX14654)
7.5% SDS PAGE
GTX107837 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169