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PGM1 antibody [AT84G2]

Anti-PGM1 antibody [AT84G2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57659
Anti-PGM1 antibody [AT84G2] used in Western Blot (WB). GTX57659

Cat. No. GTX57659

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 AT84G2

Isotype

 IgG1

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all PGM1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The clone AT84G2 is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human PGM3 protein.

Purification

Protein A Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

phosphoglucomutase 1 , CDG1T , GSD14 , PGM1

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is an isozyme of phosphoglucomutase (PGM) and belongs to the phosphohexose mutase family. There are several PGM isozymes, which are encoded by different genes and catalyze the transfer of phosphate between the 1 and 6 positions of glucose. In most cell types, this PGM isozyme is predominant, representing about 90% of total PGM activity. In red cells, PGM2 is a major isozyme. This gene is highly polymorphic. Mutations in this gene cause glycogen storage disease type 14. Alternativley spliced transcript variants encoding different isoforms have been identified in this gene.[provided by RefSeq, Mar 2010]

Database

Research Area

DATA IMAGES

Anti-PGM1 antibody [AT84G2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57659

GTX57659 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX57659 PGM1 antibody.
Blue: DAPI
Green: Primary antibody
Dilution: 1:100

Anti-PGM1 antibody [AT84G2] used in Western Blot (WB). GTX57659

GTX57659 WB Image

WB analysis of various samples using GTX57659 PGM1 antibody.
Lane 1 : 293T whole cell lysate
Lane 2 : HepG2 whole cell lysate
Lane 3 : NIH-3T3 whole cell lysate
Lane 4 : Jurkat whole cell lysate
Lane 5 : HeLa whole cell lysate
Lane 6 : U87-MG whole cell lysate
Loading : 40 μg
Dilution : 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399