GeneTex
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PGP9.5 antibody [31A3]

Anti-PGP9.5 antibody [31A3] used in Western Blot (WB). GTX34969
Anti-PGP9.5 antibody [31A3] used in IHC (Paraffin sections) (IHC-P). GTX34969

Cat No. GTX34969

Host Mouse
Clonality Monoclonal
Clone Name 31A3
Isotype IgG1
Application WB, IHC-P
Reactivity Human, Mouse, Rat, Bovine, Pig
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5-1ug/ml
IHC-P 0.5-1ug/ml for 30 minutes at RT

Note :

IHC-P
Boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes

Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Specificity/Sensitivity

This MAb reacts with a protein of 20-30kDa, identified as PGP9.5, also known as ubiquitin carboxyl-terminal hydrolase-1 (UchL1). Initially, PGP9.5 expression in normal tissues was reported in neurons and neuroendocrine cells but later it was found in distal renal tubular epithelium, spermatogonia, Leydig cells, oocytes, melanocytes, prostatic secretory epithelium, ejaculatory duct cells, epididymis, mammary epithelial cells, Merkel cells, and dermal fibroblasts. Furthermore, immunostaining for PGP9.5 has been shown in a wide variety of mesenchymal neoplasms as well. A mutation in PGP9.5 gene is believed to cause a form of Parkinson's disease.
PROPERTIES

Form

Liquid

Buffer

Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Immunogen

Native UchL1 (PGP9.5) protein from brain

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Ubiquitin C-Terminal Hydrolase L1,Hel-117,Hel-S-53,Ndgoa,Park5,Pgp 9.5,Pgp9.5,Pgp95,Spg79,Uch-L1,Uchl1

Background

The protein encoded by this gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in this gene may be associated with Parkinson disease.[provided by RefSeq, Sep 2009]

Database

Research Area

DATA IMAGES
Anti-PGP9.5 antibody [31A3] used in Western Blot (WB). GTX34969

GTX34969 WB Image

Western Blot of UchL1 (PGP9.5) in 1) human, 2) mouse and 3) rat brain Lysate using UchL1 Monoclonal Antibody (31A3).

Anti-PGP9.5 antibody [31A3] used in IHC (Paraffin sections) (IHC-P). GTX34969

GTX34969 IHC-P Image

Formalin-fixed, paraffin-embedded human Cerebellum stained with Pgp9.5 / UchL1 Monoclonal Antibody (31A3).

Package List Price ($)
$ 329