APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1-2μg/ml |
| 1-2μg/ml |
| 1-2μg/ml for 30 minutes at RT |
| 1-2μg/10⁶ cells |
|
Note :
IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
|
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
10mM PBS, 0.05% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Immunogen
Native UchL1 (PGP9.5) protein from brain
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
HEL117 , HELS53 , NDGOA , PARK5 , PGP 9.5 , PGP9.5 , PGP95 , UCHL1 , UchL1 , ubiquitin Cterminal hydrolase L1 , ubiquitin C-terminal hydrolase L1
Cellular Localization
Cytoplasm,Endoplasmic reticulum membrane
Background
The protein encoded by this gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in this gene may be associated with Parkinson disease.[provided by RefSeq, Sep 2009]
Database
Research Area
DATA IMAGES
![Anti-PGP9.5 antibody [31A3] used in Western Blot (WB). GTX34969](/templates/default/js/lightbox/images/loading.gif "Anti-PGP9.5 antibody [31A3] used in Western Blot (WB). GTX34969")
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GTX34969 WB Image
WB analysis of human brain tissue lysate using GTX34969 PGP9.5 antibody [31A3].
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GTX34969 FACS Image
FACS analysis of T98G cells using GTX34969 PGP9.5 antibody [31A3].
Blue : Primary antibody
Red : Isotype control
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GTX34969 IHC-P Image
IHC-P analysis of human brain tissue using GTX34969 PGP9.5 antibody [31A3].
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GTX34969 WB Image
WB analysis of (1) human, (2) Mouse, and (3) rat brain tissue lysates using GTX34969 PGP9.5 antibody [31A3].
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GTX34969 ICC/IF Image
ICC/IF analysis of T98G cells using GTX34969 PGP9.5 antibody [31A3].
Green : Primary antibody
Red : nucleus
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REFERENCE
There are currently no references for PGP9.5 antibody [31A3] (GTX34969). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for PGP9.5 antibody [31A3] (GTX34969). Be the first to share your experience with this product.
![Anti-PGP9.5 antibody [31A3] used in Western Blot (WB). GTX34969](/upload/website/prouct_img/small/GTX34969/GTX34969_20200115_WB_338_w_23060801_960.webp "Anti-PGP9.5 antibody [31A3] used in Western Blot (WB). GTX34969")
![Anti-PGP9.5 antibody [31A3] used in Flow cytometry (FACS). GTX34969](/upload/website/prouct_img/small/GTX34969/GTX34969_20200115_FACS_1878_w_23060801_996.webp "Anti-PGP9.5 antibody [31A3] used in Flow cytometry (FACS). GTX34969")
![Anti-PGP9.5 antibody [31A3] used in IHC (Paraffin sections) (IHC-P). GTX34969](/upload/website/prouct_img/small/GTX34969/GTX34969_20200115_IHC-P_324_w_23060801_378.webp "Anti-PGP9.5 antibody [31A3] used in IHC (Paraffin sections) (IHC-P). GTX34969")
![Anti-PGP9.5 antibody [31A3] used in Western Blot (WB). GTX34969](/upload/website/prouct_img/small/GTX34969/GTX34969_20200115_WB_3_w_23060801_608.webp "Anti-PGP9.5 antibody [31A3] used in Western Blot (WB). GTX34969")
![Anti-PGP9.5 antibody [31A3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34969](/upload/website/prouct_img/small/GTX34969/GTX34969_20200115_ICC-IF_1883_w_23060801_365.webp "Anti-PGP9.5 antibody [31A3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34969")
![Anti-PGP9.5 antibody [31A3] used in Western Blot (WB). GTX34969](/upload/website/prouct_img/normal/GTX34969/GTX34969_20200115_WB_338_w_23060801_960.webp "Anti-PGP9.5 antibody [31A3] used in Western Blot (WB). GTX34969")
![Anti-PGP9.5 antibody [31A3] used in Flow cytometry (FACS). GTX34969](/upload/website/prouct_img/normal/GTX34969/GTX34969_20200115_FACS_1878_w_23060801_996.webp "Anti-PGP9.5 antibody [31A3] used in Flow cytometry (FACS). GTX34969")
![Anti-PGP9.5 antibody [31A3] used in IHC (Paraffin sections) (IHC-P). GTX34969](/upload/website/prouct_img/normal/GTX34969/GTX34969_20200115_IHC-P_324_w_23060801_378.webp "Anti-PGP9.5 antibody [31A3] used in IHC (Paraffin sections) (IHC-P). GTX34969")
![Anti-PGP9.5 antibody [31A3] used in Western Blot (WB). GTX34969](/upload/website/prouct_img/normal/GTX34969/GTX34969_20200115_WB_3_w_23060801_608.webp "Anti-PGP9.5 antibody [31A3] used in Western Blot (WB). GTX34969")
![Anti-PGP9.5 antibody [31A3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34969](/upload/website/prouct_img/normal/GTX34969/GTX34969_20200115_ICC-IF_1883_w_23060801_365.webp "Anti-PGP9.5 antibody [31A3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34969")