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PGP9.5 antibody [3D9]

Anti-PGP9.5 antibody [3D9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57556
Anti-PGP9.5 antibody [3D9] used in Western Blot (WB). GTX57556
Anti-PGP9.5 antibody [3D9] used in IHC (Paraffin sections) (IHC-P). GTX57556

Cat. No. GTX57556

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 3D9

Isotype

 IgG2a

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all PGP9.5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
ICC/IF Assay dependent
IHC-P 1:100-1:200
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The clone 3D9 is derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human PGP9.7 protein.

Purification

Protein G Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ubiquitin C-terminal hydrolase L1 , HEL117 , HELS53 , NDGOA , PARK5 , PGP 9.5 , PGP9.5 , PGP95 , UCHL1 , UchL1 , ubiquitin Cterminal hydrolase L1

Cellular Localization

Cytoplasm,Endoplasmic reticulum membrane

Background

The protein encoded by this gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in this gene may be associated with Parkinson disease.[provided by RefSeq, Sep 2009]

Database

Research Area

DATA IMAGES

Anti-PGP9.5 antibody [3D9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57556

GTX57556 ICC/IF Image

ICC/IF analysis of U87-MG cells using GTX57556 PGP9.5 antibody.
Blue: DAPI
Green: Primary antibody
Dilution: 1:100

Anti-PGP9.5 antibody [3D9] used in Western Blot (WB). GTX57556

GTX57556 WB Image

WB analysis of various samples using GTX57556 PGP9.5 antibody.
Lane 1 : Mouse Brain lysate
Lane 2 : U87-MG whole cell lysate
Loading : 40 μg
Dilution : 1:1000

Anti-PGP9.5 antibody [3D9] used in IHC (Paraffin sections) (IHC-P). GTX57556

GTX57556 IHC-P Image

IHC-P analysis of human nerve tissue using GTX57556 PGP9.5 antibody.
Antigen retrieval: 0.1M sodium citrate buffer
Dilution: 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399