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PGP9.5 antibody [GT448]

Anti-PGP9.5 antibody [GT448] used in Western Blot (WB). GTX634797
Anti-PGP9.5 antibody [GT448] used in IHC (Paraffin sections) (IHC-P). GTX634797
Anti-PGP9.5 antibody [GT448] used in Western Blot (WB). GTX634797
Anti-PGP9.5 antibody [GT448] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634797

Cat No. GTX634797

Host

Mouse

Clonality

Monoclonal

Clone Name

GT448

Isotype

IgG1

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Positive Control

A549 , H1299

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.83 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PGP9.5. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888478

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ubiquitin C-terminal hydrolase L1 , HEL-117 , HEL-S-53 , NDGOA , PARK5 , PGP 9.5 , PGP9.5 , PGP95 , SPG79 , Uch-L1

Cellular Localization

Cytoplasm,Endoplasmic reticulum membrane

Background

The protein encoded by this gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in this gene may be associated with Parkinson disease.[provided by RefSeq, Sep 2009]

Database

Research Area

DATA IMAGES

Anti-PGP9.5 antibody [GT448] used in Western Blot (WB). GTX634797

GTX634797 WB Image

Wild-type (WT) and PGP9.5 knockout (KO) 293T cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with PGP9.5 antibody [GT448] (GTX634797) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-PGP9.5 antibody [GT448] used in IHC (Paraffin sections) (IHC-P). GTX634797

GTX634797 IHC-P Image

PGP9.5 antibody [GT448] detects PGP9.5 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
PGP9.5 stained by PGP9.5 antibody [GT448] (GTX634797) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PGP9.5 antibody [GT448] used in Western Blot (WB). GTX634797

GTX634797 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with PGP9.5 antibody [GT448] (GTX634797) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-PGP9.5 antibody [GT448] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634797

GTX634797 ICC/IF Image

PGP9.5 antibody [GT448] detects PGP9.5 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PGP9.5 stained by PGP9.5 antibody [GT448] (GTX634797) diluted at 1:500.
Red: NeuN, stained by NeuN antibody (GTX132974) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 329
$ 169