APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
A549 , H1299
Predict Reactivity
Bovine, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.83 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PGP9.5. The exact sequence is proprietary.
Purification
Affinity purified by Protein G.
Conjugation
Unconjugated
RRID
AB_2888478
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
ubiquitin C-terminal hydrolase L1 , HEL-117 , HEL-S-53 , NDGOA , PARK5 , PGP 9.5 , PGP9.5 , PGP95 , SPG79 , Uch-L1
Cellular Localization
Cytoplasm,Endoplasmic reticulum membrane
Background
The protein encoded by this gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in this gene may be associated with Parkinson disease.[provided by RefSeq, Sep 2009]
Database
Research Area
DATA IMAGES
![Anti-PGP9.5 antibody [GT448] used in Western Blot (WB). GTX634797](/templates/default/js/lightbox/images/loading.gif "Anti-PGP9.5 antibody [GT448] used in Western Blot (WB). GTX634797")
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GTX634797 WB Image
Wild-type (WT) and PGP9.5 knockout (KO) 293T cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with PGP9.5 antibody [GT448] (GTX634797) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX634797 ICC/IF Image
PGP9.5 antibody [GT448] detects PGP9.5 protein at cytoplasm by immunofluorescent analysis.Sample: mouse dorsal root ganglia cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: PGP9.5 stained by PGP9.5 antibody [GT448] (GTX634797) diluted at 1:500.Red: NeuN, a neuronal nuclei marker, stained by NeuN antibody [HL2194] (GTX638198) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX634797 IHC-P Image
PGP9.5 antibody [GT448] detects PGP9.5 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded human lung cancer.PGP9.5 stained by PGP9.5 antibody [GT448] (GTX634797) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX634797 ICC/IF Image
PGP9.5 antibody [GT448] detects PGP9.5 protein by immunofluorescent analysis.Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: PGP9.5 stained by PGP9.5 antibody [GT448] (GTX634797) diluted at 1:500.Red: NeuN, stained by NeuN antibody (GTX132974) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).
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GTX634797 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with PGP9.5 antibody [GT448] (GTX634797) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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REFERENCE
There are currently no references for PGP9.5 antibody [GT448] (GTX634797). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for PGP9.5 antibody [GT448] (GTX634797). Be the first to share your experience with this product.
![Anti-PGP9.5 antibody [GT448] used in Western Blot (WB). GTX634797](/upload/website/prouct_img/small/GTX634797/GTX634797_43339_20191115_WB_KO_watermark_w_23061202_501.webp "Anti-PGP9.5 antibody [GT448] used in Western Blot (WB). GTX634797")
![Anti-PGP9.5 antibody [GT448] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634797](/upload/website/prouct_img/small/GTX634797/GTX634797_43339_20230811_ICC_IF_M_23090619_114.webp "Anti-PGP9.5 antibody [GT448] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634797")
![Anti-PGP9.5 antibody [GT448] used in IHC (Paraffin sections) (IHC-P). GTX634797](/upload/website/prouct_img/small/GTX634797/GTX634797_43339_20190730_IHC-P_w_23061202_635.webp "Anti-PGP9.5 antibody [GT448] used in IHC (Paraffin sections) (IHC-P). GTX634797")
![Anti-PGP9.5 antibody [GT448] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634797](/upload/website/prouct_img/small/GTX634797/GTX634797_43339_20190306_ICC_IF_R_w_23061202_510.webp "Anti-PGP9.5 antibody [GT448] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634797")
![Anti-PGP9.5 antibody [GT448] used in Western Blot (WB). GTX634797](/upload/website/prouct_img/small/GTX634797/GTX634797_43339_20180921_WB_w_23061202_616.webp "Anti-PGP9.5 antibody [GT448] used in Western Blot (WB). GTX634797")
![Anti-PGP9.5 antibody [GT448] used in Western Blot (WB). GTX634797](/upload/website/prouct_img/normal/GTX634797/GTX634797_43339_20191115_WB_KO_watermark_w_23061202_501.webp "Anti-PGP9.5 antibody [GT448] used in Western Blot (WB). GTX634797")
![Anti-PGP9.5 antibody [GT448] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634797](/upload/website/prouct_img/normal/GTX634797/GTX634797_43339_20230811_ICC_IF_M_23090619_114.webp "Anti-PGP9.5 antibody [GT448] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634797")
![Anti-PGP9.5 antibody [GT448] used in IHC (Paraffin sections) (IHC-P). GTX634797](/upload/website/prouct_img/normal/GTX634797/GTX634797_43339_20190730_IHC-P_w_23061202_635.webp "Anti-PGP9.5 antibody [GT448] used in IHC (Paraffin sections) (IHC-P). GTX634797")
![Anti-PGP9.5 antibody [GT448] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634797](/upload/website/prouct_img/normal/GTX634797/GTX634797_43339_20190306_ICC_IF_R_w_23061202_510.webp "Anti-PGP9.5 antibody [GT448] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634797")
![Anti-PGP9.5 antibody [GT448] used in Western Blot (WB). GTX634797](/upload/website/prouct_img/normal/GTX634797/GTX634797_43339_20180921_WB_w_23061202_616.webp "Anti-PGP9.5 antibody [GT448] used in Western Blot (WB). GTX634797")