PRODUCT
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:100-1:200 |
|
Note :
IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 7.8 Tris-EDTA-based Target Retrieval Solution buffer.
This antibody has been validated by Autostainer including Agilent/Dako - Autostainer Link 48, Bond RX-Lecia, and Roche-Ventana Discovery ULTRA. The detailed steps by Autostainer please refer to the protocol.
|
Not tested in other applications.
Calculated MW
Positive Control
Pancreas: Islets cells and interspersed nerve fibres should show strong PGP9.5 staining.
Negative Control
Pancreas: Acinar cells and excretory ducts should not show any PGP9.5 immunostaining.
PROPERTIES
Form
Liquid
Buffer
PBS, 0.05% BSA (Please contact us for PBS only format)
Preservative
0.05% sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant full-length human UCHL1 protein
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
ubiquitin C-terminal hydrolase L1 , HEL-117 , HEL-S-53 , NDGOA , PARK5 , PGP 9.5 , PGP9.5 , PGP95 , SPG79 , Uch-L1
Cellular Localization
Cytoplasm , Endoplasmic reticulum membrane
Background
The protein encoded by this gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in this gene may be associated with Parkinson disease.[provided by RefSeq, Sep 2009]
Database
Research Area
DATA IMAGES
![Anti-PGP9.5 antibody [MSVA-905R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04438](/templates/default/js/lightbox/images/loading.gif "Anti-PGP9.5 antibody [MSVA-905R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04438")
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GTX04438 IHC-P Image
IHC-P analysis of human testis tissue using GTX04438 PGP9.5 antibody [MSVA-905R] HistoMAX™.
A strong PGP9.5 immunostaining is seen in spermatogonia. PGP9.5 staining decreases sharply in spermatocytes where it is only faint. A moderate PGP9.5 staining is seen in Leydig cells while Sertoli cells remain PGP9.5 negative.
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GTX04438 IHC-P Image
IHC-P analysis of human muscular wall from descending colon tissue using GTX04438 PGP9.5 antibody [MSVA-905R] HistoMAX™.
Strong PGP9.5 immunostaining of nerve fibres and ganglions in the muscular wall of the colon descencens.
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GTX04438 IHC-P Image
IHC-P analysis of human medullary thyroid cancer (MTC) tissue using GTX04438 PGP9.5 antibody [MSVA-905R] HistoMAX™.
Medullary thyroid cancer showing strong PGP9.5 immunostaining.
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REFERENCE
There are currently no references for PGP9.5 antibody [MSVA-905R] HistoMAX™ (GTX04438). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for PGP9.5 antibody [MSVA-905R] HistoMAX™ (GTX04438). Be the first to share your experience with this product.
![Anti-PGP9.5 antibody [MSVA-905R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04438](/upload/website/prouct_img/small/GTX04438/GTX04438_20230728_IHC-P_97_23072722_457.webp "Anti-PGP9.5 antibody [MSVA-905R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04438")
![Anti-PGP9.5 antibody [MSVA-905R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04438](/upload/website/prouct_img/small/GTX04438/GTX04438_20230728_IHC-P_227_23072723_187.webp "Anti-PGP9.5 antibody [MSVA-905R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04438")
![Anti-PGP9.5 antibody [MSVA-905R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04438](/upload/website/prouct_img/small/GTX04438/GTX04438_20230728_IHC-P_357_23072723_763.webp "Anti-PGP9.5 antibody [MSVA-905R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04438")
![Anti-PGP9.5 antibody [MSVA-905R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04438](/upload/website/prouct_img/normal/GTX04438/GTX04438_20230728_IHC-P_97_23072722_457.webp "Anti-PGP9.5 antibody [MSVA-905R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04438")
![Anti-PGP9.5 antibody [MSVA-905R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04438](/upload/website/prouct_img/normal/GTX04438/GTX04438_20230728_IHC-P_227_23072723_187.webp "Anti-PGP9.5 antibody [MSVA-905R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04438")
![Anti-PGP9.5 antibody [MSVA-905R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04438](/upload/website/prouct_img/normal/GTX04438/GTX04438_20230728_IHC-P_357_23072723_763.webp "Anti-PGP9.5 antibody [MSVA-905R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04438")