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PGP9.5 antibody [SPM574]

Anti-PGP9.5 antibody [SPM574] used in Western Blot (WB). GTX34970
Anti-PGP9.5 antibody [SPM574] used in IHC (Paraffin sections) (IHC-P). GTX34970
Anti-PGP9.5 antibody [SPM574] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34970

Cat. No. GTX34970

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 SPM574

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Bovine, Pig
Package
100 μg ($529)
See all PGP9.5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-2μg/ml
ICC/IF 1-2μg/ml
IHC-P 1-2μg/ml for 30 minutes at RT

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Native UchL1 (PGP9.5) protein from brain

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

HEL117 , HELS53 , NDGOA , PARK5 , PGP 9.5 , PGP9.5 , PGP95 , UCHL1 , UchL1 , ubiquitin Cterminal hydrolase L1 , ubiquitin C-terminal hydrolase L1

Cellular Localization

Cytoplasm,Endoplasmic reticulum membrane

Background

The protein encoded by this gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in this gene may be associated with Parkinson disease.[provided by RefSeq, Sep 2009]

Database

Research Area

DATA IMAGES

Anti-PGP9.5 antibody [SPM574] used in Western Blot (WB). GTX34970

GTX34970 WB Image

WB analysis of human brain tissue lysate using GTX34970 PGP9.5 antibody [SPM574].

Anti-PGP9.5 antibody [SPM574] used in IHC (Paraffin sections) (IHC-P). GTX34970

GTX34970 IHC-P Image

IHC-P analysis of rat cerebellum tissue using GTX34970 PGP9.5 antibody [SPM574].

Anti-PGP9.5 antibody [SPM574] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34970

GTX34970 ICC/IF Image

ICC/IF analysis of T98G cells using GTX34970 PGP9.5 antibody [SPM574].
Green : Primary antibody
Red : nucleus

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 529