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PGP9.5 antibody

Anti-PGP9.5 antibody used in Western Blot (WB). GTX101093
Anti-PGP9.5 antibody used in IHC (Paraffin sections) (IHC-P). GTX101093
Anti-PGP9.5 antibody used in Western Blot (WB). GTX101093
Anti-PGP9.5 antibody used in IHC (Frozen sections) (IHC-Fr). GTX101093
Anti-PGP9.5 antibody used in Western Blot (WB). GTX101093

Cat No. GTX101093

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, IHC-Fr
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Positive Control

A549 , H1299 , Mouse brain , rat brain

Predict Reactivity

Bovine, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.56 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PGP9.5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ubiquitin C-terminal hydrolase L1,HEL-117,HEL-S-53,NDGOA,PARK5,PGP 9.5,PGP9.5,PGP95,SPG79,Uch-L1

Cellular Localization

Cytoplasm

Background

UCHL1 is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. Expression of UCHL1 is highly specific to neurons and to cells of the diffuse neuroendocrine system and their tumors. It is present in all neurons (Doran et al., 1983 [PubMed 6343558]).[supplied by OMIM]

Database

Research Area

DATA IMAGES
Anti-PGP9.5 antibody used in Western Blot (WB). GTX101093

GTX101093 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
12% SDS PAGE
GTX101093 diluted at 1:1000

Anti-PGP9.5 antibody used in IHC (Paraffin sections) (IHC-P). GTX101093

GTX101093 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human oral cancer, using PGP9.5(GTX101093) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PGP9.5 antibody used in Western Blot (WB). GTX101093

GTX101093 WB Image

Rat tissue extract (50 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with PGP9.5 antibody (GTX101093) diluted at 1:1000.

Anti-PGP9.5 antibody used in IHC (Frozen sections) (IHC-Fr). GTX101093

GTX101093 IHC-Fr Image

PGP9.5 antibody detects PGP9.5 protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: PGP9.5 protein stained by PGP9.5 antibody (GTX101093) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-PGP9.5 antibody used in Western Blot (WB). GTX101093

GTX101093 WB Image

Whole cell extract (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with PGP9.5 antibody (GTX101093) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169