GeneTex
United States (US)

PGP9.5 antibody

KO/KD
GTX109637_39959_20160811_WB_shRNA_watermark_19011507_217.jpg
GTX109637_41101_20170503_IFA_R_19011507_696.jpg
GTX109637_41101_20160901_WB_R_brain_19011507_210.jpg
GTX109637_39959_WB_M_brain_19011507_294.jpg
GTX109637_41101_20171127_IHC-P_M_19011507_776.jpg
GTX109637_41101_WB_19011507_244.jpg
GTX109637_41101_20170531_IHC-Fr_M_2_19011507_185.jpg
GTX109637_41101_WB_M_19011507_470.jpg
GTX109637_41101_20171127_IHC-P_R_19011507_736.jpg
GTX109637_41556_IHC_R_19011507_789.jpg
GTX109637_41101_WB_R_19011507_251.jpg
GTX109637_41101_20161012_IHC-Fr_R_19011507_840.jpg

Cat No. GTX109637

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Positive Control

293T , A549 , H1299 , Neuro2A , GL261 , rat brain , PC-12 , mouse brain

Predict Reactivity

Bovine, Pig, Xenopus laevis, Xenopus tropicalis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PGP9.5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

PARK5 antibody, PGP 9.5 antibody, PGP9.5 antibody, PGP95 antibody, Uch-L1 antibody, UCHL1 antibody, ubiquitin thioesterase L1 antibody, neuron cytoplasmic protein 9.5 antibody, ubiquitin C-terminal hydrolase antibody, ubiquitin carboxyl-terminal hydrolase isozyme L1 antibody, ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase) antibody

Cellular Localization

Cytoplasm

Background

UCHL1 is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. Expression of UCHL1 is highly specific to neurons and to cells of the diffuse neuroendocrine system and their tumors. It is present in all neurons (Doran et al., 1983 [PubMed 6343558]).[supplied by OMIM]

Database

Research Area

DATA IMAGES
GTX109637_39959_20160811_WB_shRNA_watermark_19011507_217.jpg

GTX109637 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with PGP9.5 antibody (GTX109637) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX109637_41101_20170503_IFA_R_19011507_696.jpg

GTX109637 ICC/IF Image

PGP9.5 antibody detects PGP9.5 protein at cytoplasm by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PGP9.5 protein stained by PGP9.5 antibody (GTX109637) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX109637_41101_20160901_WB_R_brain_19011507_210.jpg

GTX109637 WB Image

Rat tissue extract (50 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with PGP9.5 antibody (GTX109637) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX109637_39959_WB_M_brain_19011507_294.jpg

GTX109637 WB Image

PGP9.5 antibody detects UCHL1 protein by western blot analysis.
A. 50 µg mouse brain lysate/extract
12% SDS-PAGE
PGP9.5 antibody (GTX109637) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX109637_41101_20171127_IHC-P_M_19011507_776.jpg

GTX109637 IHC-P Image

PGP9.5 antibody detects PGP9.5 protein by immunohistochemical analysis.
Samples: Paraffin-embedded mouse skin.
Green: PGP9.5 protein stained by PGP9.5 antibody (GTX109637) diluted at 1:250.
Red: beta Tubulin 3/ Tuj1, a marker, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX109637_41101_WB_19011507_244.jpg

GTX109637 WB Image

PGP9.5 antibody detects PGP9.5 protein by western blot analysis.
A. 30 µg A549 whole cell lysate/extract
B. 30 µg H1299 whole cell lysate/extract
12% SDS-PAGE
PGP9.5 antibody (GTX109637) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX109637_41101_20170531_IHC-Fr_M_2_19011507_185.jpg

GTX109637 IHC-Fr Image

PGP9.5 antibody detects PGP9.5 protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: PGP9.5 protein stained by PGP9.5 antibody (GTX109637) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX109637_41101_WB_M_19011507_470.jpg

GTX109637 WB Image

PGP9.5 antibody detects PGP9.5 protein by western blot analysis.
A. 30 µg Neuro2A whole cell lysate/extract
B. 30 µg GL261 whole cell lysate/extract
12% SDS-PAGE
PGP9.5 antibody (GTX109637) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX109637_41101_20171127_IHC-P_R_19011507_736.jpg

GTX109637 IHC-P Image

PGP9.5 antibody detects PGP9.5 protein by immunohistochemical analysis.
Samples: Paraffin-embedded rat colon.
Green: PGP9.5 protein stained by PGP9.5 antibody (GTX109637) diluted at 1:250.
Red: beta Tubulin 3/ Tuj1, a marker, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX109637_41556_IHC_R_19011507_789.jpg

GTX109637 IHC-P Image

PGP9.5 antibody detects PGP9.5 protein at cytosol on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
PGP9.5 antibody (GTX109637) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX109637_41101_WB_R_19011507_251.jpg

GTX109637 WB Image

PGP9.5 antibody detects PGP9.5 protein by western blot analysis.
A. 30 µg PC-12 whole cell lysate/extract
12% SDS-PAGE
PGP9.5 antibody (GTX109637) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX109637_41101_20161012_IHC-Fr_R_19011507_840.jpg

GTX109637 IHC-Fr Image

PGP9.5 antibody detects PGP9.5 protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: PGP9.5 protein stained by PGP9.5 antibody (GTX109637) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169