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PGP9.5 antibody

Anti-PGP9.5 antibody used in Western Blot (WB). GTX109646
Anti-PGP9.5 antibody used in Western Blot (WB). GTX109646
Anti-PGP9.5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109646
Anti-PGP9.5 antibody used in Western Blot (WB). GTX109646
Anti-PGP9.5 antibody used in IHC (Paraffin sections) (IHC-P). GTX109646
Anti-PGP9.5 antibody used in Western Blot (WB). GTX109646
Anti-PGP9.5 antibody used in Western Blot (WB). GTX109646
Anti-PGP9.5 antibody used in IHC (Frozen sections) (IHC-Fr). GTX109646
Anti-PGP9.5 antibody used in IHC (Paraffin sections) (IHC-P). GTX109646
Anti-PGP9.5 antibody used in IHC (Frozen sections) (IHC-Fr). GTX109646

Cat No. GTX109646

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Positive Control

293T , A549 , H1299 , mouse brain , rat brain

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PGP9.5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952495

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ubiquitin C-terminal hydrolase L1 , HEL-117 , HEL-S-53 , NDGOA , PARK5 , PGP 9.5 , PGP9.5 , PGP95 , SPG79 , Uch-L1

Cellular Localization

Cytoplasm

Background

UCHL1 is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. Expression of UCHL1 is highly specific to neurons and to cells of the diffuse neuroendocrine system and their tumors. It is present in all neurons (Doran et al., 1983 [PubMed 6343558]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-PGP9.5 antibody used in Western Blot (WB). GTX109646

GTX109646 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with PGP9.5 antibody (GTX109646) diluted at 1:3000.

Anti-PGP9.5 antibody used in Western Blot (WB). GTX109646

GTX109646 WB Image

Wild-type (WT) and PGP9.5 knockout (KO) 293T cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with PGP9.5 antibody (GTX109646) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PGP9.5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109646

GTX109646 ICC/IF Image

PGP9.5 antibody detects PGP9.5 protein at cytoplasm by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PGP9.5 protein stained by PGP9.5 antibody (GTX109646) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-PGP9.5 antibody used in Western Blot (WB). GTX109646

GTX109646 WB Image

Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with PGP9.5 antibody (GTX109646) diluted at 1:10000.

Anti-PGP9.5 antibody used in IHC (Paraffin sections) (IHC-P). GTX109646

GTX109646 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SEROUS OVCA xenograft, using PGP9.5(GTX109646) antibody at 1:300 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PGP9.5 antibody used in Western Blot (WB). GTX109646

GTX109646 WB Image

Sample (30 ug of whole cell lysate)
A: A549
B: H1299
12% SDS PAGE
GTX109646 diluted at 1:5000

Anti-PGP9.5 antibody used in Western Blot (WB). GTX109646

GTX109646 WB Image

PGP9.5 antibody detects UCHL1 protein by Western blot analysis.
A. 50 μg mouse brain lysate/extract
12 % SDS-PAGE
PGP9.5 antibody (GTX109646) dilution: 1:5000

Anti-PGP9.5 antibody used in IHC (Frozen sections) (IHC-Fr). GTX109646

GTX109646 IHC-Fr Image

PGP9.5 antibody detects PGP9.5 protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: PGP9.5 protein stained by PGP9.5 antibody (GTX109646) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-PGP9.5 antibody used in IHC (Paraffin sections) (IHC-P). GTX109646

GTX109646 IHC-P Image

PGP9.5 antibody detects PGP9.5 protein by immunohistochemical analysis.
Samples: Paraffin-embedded rat colon.
Green: PGP9.5 protein stained by PGP9.5 antibody (GTX109646) diluted at 1:250.
Red: beta Tubulin 3/ Tuj1, a marker, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PGP9.5 antibody used in IHC (Frozen sections) (IHC-Fr). GTX109646

GTX109646 IHC-Fr Image

PGP9.5 antibody detects PGP9.5 protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: PGP9.5 protein stained by PGP9.5 antibody (GTX109646) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169