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PHC1 antibody [1F3F3]

Anti-PHC1 antibody [1F3F3] used in ELISA (ELISA). GTX60752
Anti-PHC1 antibody [1F3F3] used in Flow cytometry (FACS). GTX60752
Anti-PHC1 antibody [1F3F3] used in Western Blot (WB). GTX60752

Cat. No. GTX60752

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1F3F3

Isotype

 IgG1

Application

 WB, FACS, ELISA

Reactivity

 Human
Package
100 μg ($409)
See all PHC1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

106 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant fragment of human PHC1 (AA: 758-1004) expressed in E. Coli.

Purification

Protein G Purified

Conjugation

Unconjugated

RRID

AB_2888000

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

polyhomeotic homolog 1 , EDR1 , HPH1 , MCPH11 , RAE28

Cellular Localization

Nucleus

Background

This gene is a homolog of the Drosophila polyhomeotic gene, which is a member of the Polycomb group of genes. The gene product is a component of a multimeric protein complex that contains EDR2 and the vertebrate Polycomb protein BMH1. The gene product, the EDR2 protein, and the Drosophila polyhomeotic protein share 2 highly conserved domains, named homology domains I and II. These domains are involved in protein-protein interactions and may mediate heterodimerization of the protein encoded by this gene and the EDR2 protein. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-PHC1 antibody [1F3F3] used in ELISA (ELISA). GTX60752

GTX60752 ELISA Image

ELISA analysis of antigen using GTX60752 PHC1 antibody [1F3F3].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

Anti-PHC1 antibody [1F3F3] used in Flow cytometry (FACS). GTX60752

GTX60752 FACS Image

FACS analysis of HEK293 cells using GTX60752 PHC1 antibody [1F3F3].
Green : PHC1
Red : negative control

Anti-PHC1 antibody [1F3F3] used in Western Blot (WB). GTX60752

GTX60752 WB Image

WB analysis of HEK293 (1) and PHC1 (AA: 758-1004)-hIgGFc transfected HEK293 (2) cell lysate using GTX60752 PHC1 antibody [1F3F3].

REFERENCE

Application Reference

Liu K et al. Proc Natl Acad Sci U S A 2014; 111 (38) : 13960-5 Neuronal necrosis is regulated by a conserved chromatin-modifying cascade.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 409