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PHD1 antibody

Anti-PHD1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX123033
Anti-PHD1 antibody used in Western Blot (WB). GTX123033
Anti-PHD1 antibody used in IHC (Paraffin sections) (IHC-P). GTX123033

Cat No. GTX123033

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Positive Control

A549 , HeLa , HepG2 , HCT116

Predict Reactivity

Mouse, Rat, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PHD1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Egl-9 Family Hypoxia Inducible Factor 2,Eit-6,Eit6,Hif-Ph1,Hifph1,Hph-1,Hph-3,Phd1,Egln2

Cellular Localization

Cytoplasm , Nucleus

Background

The hypoxia inducible factor (HIF) is a transcriptional complex which is involved in oxygen homeostasis. At normal oxygen levels, the alpha subunit of HIF is targeted for degration by prolyl hydroxylation. This gene encodes an enzyme responsible for this posttranslational modification. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-PHD1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX123033

GTX123033 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using PHD1(GTX123033) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-PHD1 antibody used in Western Blot (WB). GTX123033

GTX123033 WB Image

Sample (30 µg of whole cell lysate)
A: HeLa
10% SDS PAGE
GTX123033 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PHD1 antibody used in IHC (Paraffin sections) (IHC-P). GTX123033

GTX123033 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma, using PHD1(GTX123033) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf