*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
A549 , HeLa , HepG2 , HCT116
Mouse, Rat, Xenopus laevis, Chimpanzee, Rhesus Monkey(>80% identity)
1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1mg/ml(Please refer to the vial label for the specific concentration.)
Recombinant protein encompassing a sequence within the center region of human PHD1. The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
DKFZp434E026 antibody, EIT6 antibody, FLJ95603 antibody, HIF-PH1 antibody, HIFPH1 antibody, HPH-1 antibody, HPH-3 antibody, PHD1 antibody, EGLN2 antibody, egl nine homolog 2 antibody, hypoxia-inducible factor prolyl hydroxylase 1 antibody, estrogen-induced tag 6 antibody, HIF-prolyl hydroxylase 1 antibody, prolyl hydroxylase domain-containing protein 1 antibody, egl nine homolog 2 (C. elegans) antibody
Cytoplasm , Nucleus
The hypoxia inducible factor (HIF) is a transcriptional complex which is involved in oxygen homeostasis. At normal oxygen levels, the alpha subunit of HIF is targeted for degration by prolyl hydroxylation. This gene encodes an enzyme responsible for this posttranslational modification. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq]
GTX123033 ICC/IF Image
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using PHD1(GTX123033) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
GTX123033 WB Image
Sample (30 µg of whole cell lysate)
10% SDS PAGE
GTX123033 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX123033 IHC-P Image
Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma, using PHD1(GTX123033) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min