GeneTex
United States (US)

PHD1 antibody

GTX64646_20180530_WB_18121411_654.jpg
GTX64646_20180530_IP_18121411_814.jpg

Cat No. GTX64646

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IP
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
IP 1:20 - 1:50
Not tested in other applications.

Calculated MW

44 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 150-250 of human EGLN2 (NP_444274.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

HPH1 Antibody , HIF PH1 Antibody , EGLN2 Antibody , EIT6 Antibody , HPH3 Antibody , HIFPH1 Antibody

Background

The hypoxia inducible factor (HIF) is a transcriptional complex that is involved in oxygen homeostasis. At normal oxygen levels, the alpha subunit of HIF is targeted for degration by prolyl hydroxylation. This gene encodes an enzyme responsible for this post-translational modification. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the upstream RAB4B (RAB4B, member RAS oncogene family) gene. [provided by RefSeq, Feb 2011]

Database

Research Area

DATA IMAGES
GTX64646_20180530_WB_18121411_654.jpg

GTX64646 WB Image

WB analysis of various samples using GTX64646 PHD1 antibody.
Dilution : 1:1000
Loading : 25μg per lane

GTX64646_20180530_IP_18121411_814.jpg

GTX64646 IP Image

IP analysis of HeLa cell lysate using GTX64646 PHD1 antibody.
IP reaction : 3μg antibody / 150μg cell lysate
Dilution : 1:1000