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PHD2 antibody [AT21E9]

Anti-PHD2 antibody [AT21E9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50009
Anti-PHD2 antibody [AT21E9] used in Western Blot (WB). GTX50009

Cat No. GTX50009

Host

Mouse

Clonality

Monoclonal

Clone Name

AT21E9

Isotype

IgG2b

Application

WB, ICC/IF, ELISA

Reactivity

Human
Package
100 μl ($319)

APPLICATION

Application Note

We recommend the following starting dilutions:
Western blot and Immunofluorescence analysis: Use at 1:500 ~ 1000. Recommended starting dilution is 1:500.

Optimal working concentrations should be determined experimentally by the end user.

Calculated MW

46 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

In Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant EGLN1 (271-426aa) purified from E. coli

Purification

By protein-G affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Egl-9 Family Hypoxia Inducible Factor 1 , C1Orf12 , Ecyt3 , Halah , Hif-Ph2 , Hifph2 , Hph-2 , Hph2 , Phd2 , Sm20 , Zmynd6 , Egln1

Background

PHD1 (Egln2), PHD-2 (Egln1), and PHD3 (Egln3) are members of the Egln family of proline hydroxylases. EGLN family, which is known to be cellular oxygen sensor, initiates the first step of HIF degradation through the VHL/ubiquitin pathway. PHD1 and PHD2 can hydroxylate both proline 402 and proline 564, but PHD3 can only hydroxylate proline 564. In addition to HIF, PHD enzymes have also has been shown to catalyze the hydroxylation of RNA polymerase subunits and myogenin.

Database

Research Area

DATA IMAGES

Anti-PHD2 antibody [AT21E9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50009

GTX50009 ICC/IF Image

HeLa cells were stained with monoclonal anti-EGLN1 antibody (Green). Nucleus and Actin were stained by Phalloidin-TRITC (Red) or Hoechst 33342 (Blue).

Anti-PHD2 antibody [AT21E9] used in Western Blot (WB). GTX50009

GTX50009 WB Image

Cell lysates of HeLa (35ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human EGLN1 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf