APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500 - 1:2500 |
| Assay dependent |
| Assay dependent |
| 1:10 - 1:1000 |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Calculated MW
Product Note
We do not recommend use of this product for Mouse samples.
PROPERTIES
Form
Liquid
Buffer
Tris-Citrate/Phosphate
Preservative
0.09% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Synthetic peptide made to a C-terminal portion of human PHD2/HIF Prolyl Hydroxylase 2 (between amino acids 350-426) (GeneID 54583).
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
egl-9 family hypoxia inducible factor 1 , C1orf12 , ECYT3 , HALAH , HIF-PH2 , HIFPH2 , HPH-2 , HPH2 , PHD2 , SM20 , ZMYND6
Cellular Localization
Cytoplasm,Nucleus
Background
The protein encoded by this gene catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. HIF is a transcriptional complex that plays a central role in mammalian oxygen homeostasis. This protein functions as a cellular oxygen sensor, and under normal oxygen concentration, modification by prolyl hydroxylation is a key regulatory event that targets HIF subunits for proteasomal destruction via the von Hippel-Lindau ubiquitylation complex. Mutations in this gene are associated with erythrocytosis familial type 3 (ECYT3). [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX10930 FACS Image
FACS analysis of 10⁶ Jurkat cells (fixed and permeabilized) in 150μl reaction using GTX10930 PHD2 antibody.
Dilution : 3 μg/ml
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GTX10930 WB Image
WB analysis of DDDDK-his-PHD1, PHD2, PHD3 recombinant protein, (10 ng/lane), hypoxia-treated HeLa and MEF cell lysate using GTX10930 PHD2 antibody.
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GTX10930 WB Image
WB analysis of epitope-tagged PHD1, PHD2 or PHD3 (10 ng/lane) and hypoxia-treated HeLa cells lysate using GTX10930 PHD2 antibody.
Dilution : 1 μg/ml
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REFERENCE
There are currently no references for PHD2 antibody (GTX10930). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for PHD2 antibody (GTX10930). Be the first to share your experience with this product.
