GeneTex

  • Country / Location Selection

United States (US)

PHD2 antibody

Orthogonal
Anti-PHD2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132293
Anti-PHD2 antibody used in Western Blot (WB). GTX132293
Anti-PHD2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132293
Anti-PHD2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX132293

Cat. No. GTX132293

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all PHD2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Positive Control

HeLa(200 μM CoCl2 treatment for 24 hr)

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.99 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PHD2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886598

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

egl-9 family hypoxia inducible factor 1 , C1orf12 , ECYT3 , HALAH , HIF-PH2 , HIFPH2 , HPH-2 , HPH2 , PHD2 , SM20 , ZMYND6

Background

The protein encoded by this gene catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. HIF is a transcriptional complex that plays a central role in mammalian oxygen homeostasis. This protein functions as a cellular oxygen sensor, and under normal oxygen concentration, modification by prolyl hydroxylation is a key regulatory event that targets HIF subunits for proteasomal destruction via the von Hippel-Lindau ubiquitylation complex. Mutations in this gene are associated with erythrocytosis familial type 3 (ECYT3). [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PHD2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132293

GTX132293 ICC/IF Image

PHD2 antibody detects PHD2 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PHD2 stained by PHD2 antibody (GTX132293) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-PHD2 antibody used in Western Blot (WB). GTX132293

GTX132293 WB Image

Orthogonal

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PHD2 antibody (GTX132293) diluted at 1:2000.

Anti-PHD2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132293

GTX132293 ICC/IF Image

PHD2 antibody detects PHD2 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PHD2 stained by PHD2 antibody (GTX132293) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-PHD2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX132293

GTX132293 IHC-Fr Image

PHD2 antibody detects PHD2 Protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: PHD2 stained by PHD2 antibody (GTX132293) diluted at 1:250.
Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 10 min

REFERENCE

Application Reference

Ho YJ et al. Theranostics 2019; 9 (24) : 7370-7383 Normalization of Tumor Vasculature by Oxygen Microbubbles with Ultrasound.
Application : WB
Reactivity : Mouse
Ma CP et al. EMBO Rep 2019; 20 (5) ADAR1 promotes robust hypoxia signaling via distinct regulation of multiple HIF-1α-inhibiting factors.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

There are currently no reviews for PHD2 antibody (GTX132293). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169