GeneTex
United States (US)

PHD2 antibody

Orthogonal
GTX132293_42340_20170831_IHC-Fr_M_18121410_102.jpg
GTX132293_42340_20171115_ICC_IF_R_18121410_221.jpg
GTX132293_42340_20161006_WB_CoCl2_18121410_347.jpg

Cat No. GTX132293

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-Fr
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Positive Control

HeLa , HeLa(200 μM CoCl2 treatment for 24 hr)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.99mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PHD2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

C1orf12 antibody , ECYT3 antibody , EGLN1 antibody , HIF PH2 antibody , HIFPH2 antibody , HPH2 antibody , SM20 antibody , ZMYND6 antibody , egl-9 family hypoxia-inducible factor 1 antibody

Background

The protein encoded by this gene catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. HIF is a transcriptional complex that plays a central role in mammalian oxygen homeostasis. This protein functions as a cellular oxygen sensor, and under normal oxygen concentration, modification by prolyl hydroxylation is a key regulatory event that targets HIF subunits for proteasomal destruction via the von Hippel-Lindau ubiquitylation complex. Mutations in this gene are associated with erythrocytosis familial type 3 (ECYT3). [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX132293_42340_20170831_IHC-Fr_M_18121410_102.jpg

GTX132293 IHC-Fr Image

PHD2 antibody detects PHD2 Protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: PHD2 stained by PHD2 antibody (GTX132293) diluted at 1:250.
Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 10 min

GTX132293_42340_20171115_ICC_IF_R_18121410_221.jpg

GTX132293 ICC/IF Image

PHD2 antibody detects PHD2 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PHD2 stained by PHD2 antibody (GTX132293) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX132293_42340_20161006_WB_CoCl2_18121410_347.jpg

GTX132293 WB Image

Orthogonal

Untreated (–) and treated (+) HeLa whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with PHD2 antibody (GTX132293) diluted at 1:2000.

SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf