APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
2 μg/ml |
1:50 - 1:500 |
2.5 - 5.0 μg/ml |
1:10 - 1:500 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Mouse
Immunogen
A synthetic peptide made to an internal portion of mouse PHD2/HIF Prolyl Hydroxylase 2 (between residues 300-400). [UniProt# Q91YE3]
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
egl-9 family hypoxia-inducible factor 1 , AI503754 , C1orf12 , HIF-PH2 , HPH-2 , Hif-p4h-2 , ORF13 , Phd2 , SM-20
Cellular Localization
Cytoplasm,Nucleus
Background
Catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF-1 alpha at 'Pro-402' and 'Pro-564', and HIF-2 alpha. Functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex.
Database
Research Area
DATA IMAGES
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GTX82966 IHC-P Image
IHC-P analysis of mouse kidney tissue using GTX82966 PHD2 antibody. Dilution : 2.5 μg/ml
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GTX82966 WB Image
WB analysis of mouse kidney tissue using GTX82966 PHD2 antibody.
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GTX82966 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX82966 PHD2 antibody. Green : primary antibody Red : Tubulin Blue : DAPI Dilution : 1:500
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GTX82966 IHC-P Image
IHC-P analysis of mouse lung tissue using GTX82966 PHD2 antibody. Dilution : 1:200
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REFERENCE
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REVIEW
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