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PHD3 antibody

Anti-PHD3 antibody used in Western Blot (WB). GTX10880
Anti-PHD3 antibody used in Western Blot (WB). GTX10880
Anti-PHD3 antibody used in IHC (Paraffin sections) (IHC-P). GTX10880
Anti-PHD3 antibody used in Western Blot (WB). GTX10880
Anti-PHD3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX10880

Cat. No. GTX10880

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP, ChIP assay

Reactivity

 Human, Mouse, Rat, Primate
Package
100 μl ($419)
See all PHD3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF Assay dependent
IHC-P Assay dependent
IP 1:10 - 1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

27 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide made to the C-terminus of humanPHD3/HIF Prolyl Hydroxylase 3. [LocusLink ID 112399]

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

egl-9 family hypoxia inducible factor 3 , HIFP4H3 , HIFPH3 , PHD3

Cellular Localization

Nucleus,Cytoplasm

Background

Catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF-1 alpha at 'Pro-564', and HIF-2 alpha. Functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. May play a role in cell growth regulation in muscle cells and in apoptosis in neuronal tissue. Promotes cell death through a caspase-dependent mechanism.

Database

Research Area

DATA IMAGES

Anti-PHD3 antibody used in Western Blot (WB). GTX10880

GTX10880 WB Image

WB analysis of MEF cell lysate using GTX10880 PHD3 antibody.
Dilution : 2 μg/ml

Anti-PHD3 antibody used in Western Blot (WB). GTX10880

GTX10880 WB Image

WB analysis of MEF, RT4, HeLa, and PC12 cell lysate using GTX10880 PHD3 antibody.
Dilution : 2 μg/ml

Anti-PHD3 antibody used in IHC (Paraffin sections) (IHC-P). GTX10880

GTX10880 IHC-P Image

IHC-P analysis of human liver cancer tissue using GTX10880 PHD3 antibody.
Dilution : 1:3000

Anti-PHD3 antibody used in Western Blot (WB). GTX10880

GTX10880 WB Image

WB analysis of hypoxic-treated HeLa cell lysate using GTX10880 PHD3 antibody.

Anti-PHD3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX10880

GTX10880 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX10880 PHD3 antibody.
Green : primary antibody
Red : Tubulin
Blue : DAPI
Dilution : 1:200
Fixation : 10% formalin
Permibilization : 0.5% Triton-X100

REFERENCE

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 419