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PHD4 antibody [N2C2], Internal

Anti-PHD4 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX116581
Anti-PHD4 antibody [N2C2], Internal used in Western Blot (WB). GTX116581

Cat. No. GTX116581

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all PHD4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Positive Control

A549 , H1299 , HCT116

Predict Reactivity

Mouse, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PHD4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11176582

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

prolyl 4-hydroxylase, transmembrane , EGLN4 , HIFPH4 , P4H-TM , PH-4 , PH4 , PHD4

Cellular Localization

Endoplasmic reticulum membrane; Single-pass type II membrane protein

Background

The product of this gene belongs to the family of prolyl 4-hydroxylases. This protein is a prolyl hydroxylase that may be involved in the degradation of hypoxia-inducible transcription factors under normoxia. It plays a role in adaptation to hypoxia and may be related to cellular oxygen sensing. Alternatively spliced variants encoding different isoforms have been identified. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PHD4 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX116581

GTX116581 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using PHD4(GTX116581) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PHD4 antibody [N2C2], Internal used in Western Blot (WB). GTX116581

GTX116581 WB Image

Sample (30 ug of whole cell lysate)
A: A549
B: H1299
C: HCT116
7.5% SDS PAGE
GTX116581 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169