GeneTex
United States (US)

PHD4 antibody

GTX116582_40268_20150624_IHC-P_18121410_633.jpg
GTX116582_WB_18121410_375.jpg

Cat No. GTX116582

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Positive Control

A549 , HeLa

Predict Reactivity

Mouse, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.88mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PHD4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

EGLN4 antibody, HIFPH4 antibody, P4H-TM antibody, PH-4 antibody, PH4 antibody, PHD4 antibody, P4HTM antibody, hypoxia inducible factor prolyl 4 hydroxylase antibody, proline 4-hydroxylase antibody, hypoxia-inducible factor prolyl hydroxylase 4 antibody, HIF-prolyl hydroxylase 4 antibody, P4H with transmembrane domain antibody, hypoxia-inducible factor prolyl 4-hydroxylase antibody, Prolyl hydroxlase domain-containing 4 antibody, HIF-PH4 antibody, HPH-4 antibody, transmembrane prolyl 4-hydroxylase antibody, "prolyl 4-hydroxylase, transmembrane (endoplasmic reticulum) antibody"

Background

The product of this gene belongs to the family of prolyl 4-hydroxylases. This protein is a prolyl hydroxylase that may be involved in the degradation of hypoxia-inducible transcription factors under normoxia. It plays a role in adaptation to hypoxia and may be related to cellular oxygen sensing. Alternatively spliced variants encoding different isoforms have been identified. [provided by RefSeq]

Database

DATA IMAGES
GTX116582_40268_20150624_IHC-P_18121410_633.jpg

GTX116582 IHC-P Image

PHD4 antibody detects PHD4 protein at cytoplasm in human lung cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
PHD4 antibody (GTX116582) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX116582_WB_18121410_375.jpg

GTX116582 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
7.5% SDS PAGE
GTX116582 diluted at 1:2000

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169