GeneTex
  • Country / Location Selection

United States (US)

PHD4 antibody

Anti-PHD4 antibody used in IHC (Paraffin sections) (IHC-P). GTX116582
Anti-PHD4 antibody used in Western Blot (WB). GTX116582

Cat No. GTX116582

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Positive Control

A549 , HeLa

Predict Reactivity

Mouse, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.88 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PHD4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

prolyl 4-hydroxylase, transmembrane,EGLN4,HIFPH4,P4H-TM,PH-4,PH4,PHD4

Background

The product of this gene belongs to the family of prolyl 4-hydroxylases. This protein is a prolyl hydroxylase that may be involved in the degradation of hypoxia-inducible transcription factors under normoxia. It plays a role in adaptation to hypoxia and may be related to cellular oxygen sensing. Alternatively spliced variants encoding different isoforms have been identified. [provided by RefSeq]

Database

DATA IMAGES
Anti-PHD4 antibody used in IHC (Paraffin sections) (IHC-P). GTX116582

GTX116582 IHC-P Image

PHD4 antibody detects PHD4 protein at cytoplasm in human lung cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
PHD4 antibody (GTX116582) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PHD4 antibody used in Western Blot (WB). GTX116582

GTX116582 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
7.5% SDS PAGE
GTX116582 diluted at 1:2000

REFERENCE
There are currently no references for PHD4 antibody (GTX116582). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for PHD4 antibody (GTX116582). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169