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PHF10 antibody

Orthogonal
Anti-PHF10 antibody used in Western Blot (WB). GTX116314
Anti-PHF10 antibody used in IHC (Paraffin sections) (IHC-P). GTX116314
Anti-PHF10 antibody used in IHC (Paraffin sections) (IHC-P). GTX116314
Anti-PHF10 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116314
Anti-PHF10 antibody used in Western Blot (WB). GTX116314
Anti-PHF10 antibody used in IHC (Paraffin sections) (IHC-P). GTX116314

Cat. No. GTX116314

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all PHF10 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Positive Control

mouse colon

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.06 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PHF10. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11176023

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

PHD finger protein 10 , BAF45A , XAP135

Cellular Localization

Nucleus

Background

This gene contains a predicted ORF that encodes a protein with two zinc finger domains. The function of the encoded protein is not known. Sequence analysis suggests that multiple alternatively spliced transcript variants are derived from this gene but the full-length nature of only two of them is known. These two splice variants encode different isoforms. A pseudogene for this gene is located on Xq28. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-PHF10 antibody used in Western Blot (WB). GTX116314

GTX116314 WB Image

Orthogonal

HeLa whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PHF10 antibody (GTX116314) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PHF10 antibody used in IHC (Paraffin sections) (IHC-P). GTX116314

GTX116314 IHC-P Image

PHF10 antibody detects PHF10 protein at nucleus in rat lung by immunohistochemical analysis.
Sample: Paraffin-embedded rat lung.
PHF10 antibody (GTX116314) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-PHF10 antibody used in IHC (Paraffin sections) (IHC-P). GTX116314

GTX116314 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Hela xenograft, using PHF10(GTX116314) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PHF10 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116314

GTX116314 ICC/IF Image

PHF10 antibody detects PHF10 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: PHF10 protein stained by PHF10 antibody (GTX116314) diluted at 1:500.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:100.
Blue: Hoechst 33342 staining.
Anti-PHF10 antibody used in Western Blot (WB). GTX116314

GTX116314 WB Image

PHF10 antibody detects PHF10 protein by western blot analysis.
A.50 μg mouse colon lysate/extract
10% SDS-PAGE
PHF10 antibody (GTX116314) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PHF10 antibody used in IHC (Paraffin sections) (IHC-P). GTX116314

GTX116314 IHC-P Image

PHF10 antibody detects PHF10 protein at nucleus in mouse lung by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
PHF10 antibody (GTX116314) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Z Fan et al. Heliyon 2024; 10 (7) : e29109 PHD finger protein 10 promotes cell proliferation by regulating CD44 transcription in gastric cancer
Application : WB, IHC-P
Zhang Y et al. Brain Res Bull 2022; 181 : 12-20 Treadmill training attenuate STZ-induced cognitive dysfunction in type 2 diabetic rats via modulating Grb10/IGF-R signaling.
Shi H et al. Sci Adv 2020; 6 (29) : eaaz3440 ARID1A loss in neuroblastoma promotes the adrenergic-to-mesenchymal transition by regulating enhancer-mediated gene expression.
Application : WB
Wei Z et al. Cell 2018; 173 (5) : 1135-1149.e15 Vitamin D Switches BAF Complexes to Protect β Cells.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169