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PHF2 antibody

Anti-PHF2 antibody used in Western Blot (WB). GTX129028

Cat. No. GTX129028

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all PHF2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

121 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PHF2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885875

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

PHD finger protein 2 , CENP-35 , GRC5 , JHDM1E , KDM7C

Cellular Localization

Nucleus

Background

Probable regulator of histone methyltransferase complexes. Recruited to trimethylated 'Lys-4' of histone H3 (H3K4me3) at rDNA promoters and promotes expression of rDNA. Was reported to have histone demethylase activity by itself and specifically demethylate monomethylated 'Lys-9' of histone H3 (H3K9me1), an epigenetic repressive mark. However, this activity is unclear in vivo and requires additional evidence.

Database

Research Area

DATA IMAGES

Anti-PHF2 antibody used in Western Blot (WB). GTX129028

GTX129028 WB Image

PHF2 antibody detects PHF2 protein by western blot analysis.
A. 30 μg 293T whole cell extract
B. 30 μg A431 whole cell extract
C. 30 μg HeLa whole cell extract
D. 30 μg HepG2 whole cell extract
5 % SDS-PAGE
PHF2 antibody (GTX129028) dilution: 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169