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PHF5A antibody

Anti-PHF5A antibody used in Flow cytometry (FACS). GTX03384
Anti-PHF5A antibody used in Western Blot (WB). GTX03384

Cat. No. GTX03384

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, FACS

Reactivity

 Human
Package
400 μl ($399)
See all PHF5A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
FACS Assay dependent
Not tested in other applications.

Calculated MW

12 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide between 81-110 amino acids from the C-terminal region of human PHF5A.

Purification

Protein A purified followed by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

PHD finger protein 5A , INI , Rds3 , SAP14b , SF3B7 , SF3b14b , bK223H9.2

Cellular Localization

Nucleus , Nucleus speckle

Background

This gene encodes a subunit of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence-independent manner and may anchor the U2 snRNP to the pre-mRNA. The protein encoded by this gene contains a PHD-finger-like domain that is flanked by highly basic N- and C-termini. This protein belongs to the PHD-finger superfamily and may act as a chromatin-associated protein. This gene has several pseudogenes on different chromosomes. [provided by RefSeq, Jul 2008]

Database

DATA IMAGES

Anti-PHF5A antibody used in Flow cytometry (FACS). GTX03384

GTX03384 FACS Image

FACS analysis of HeLa cells (right histogram) compared to a negative control cell (left histogram) using GTX03384 PHF5A antibody.

Anti-PHF5A antibody used in Western Blot (WB). GTX03384

GTX03384 WB Image

WB analysis of various sample lysates using GTX03384 PHF5A antibody.
Lane 1 : HeLa cell
Lane 2 : HL-60 cell
Dilution : 1:1000
Loading : 20 μg per lane

REFERENCE

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REVIEW

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Package List Price ($)
$ 399