MCF-7 whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PHF6 antibody [N2C2], Internal (GTX116966) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunofluorescence analysis of paraformaldehyde-fixed MCF-7, using PHF6(GTX116966) antibody at 1:500 dilution.