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PHGDH antibody [N1N2], N-term

Anti-PHGDH antibody [N1N2], N-term used in Western Blot (WB). GTX101948
Anti-PHGDH antibody [N1N2], N-term used in Immunoprecipitation (IP). GTX101948
Anti-PHGDH antibody [N1N2], N-term used in IHC (Frozen sections) (IHC-Fr). GTX101948
Anti-PHGDH antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101948
Anti-PHGDH antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX101948
Anti-PHGDH antibody [N1N2], N-term used in Western Blot (WB). GTX101948
Anti-PHGDH antibody [N1N2], N-term used in Western Blot (WB). GTX101948
Anti-PHGDH antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX101948

Cat No. GTX101948

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr, IP
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Positive Control

HeLa , 293T , A431 , HeLaS3 , mouse brain , *SK-N-DZ

Predict Reactivity

Bovine, Pig, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.87 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human PHGDH. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

phosphoglycerate dehydrogenase , 3-PGDH , 3PGDH , HEL-S-113 , NLS , NLS1 , PDG , PGAD , PGD , PGDH , PHGDHD , SERA

Background

3-Phosphoglycerate dehydrogenase (PHGDH; EC 1.1.1.95) catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate, which is the first and rate-limiting step in the phosphorylated pathway of serine biosynthesis, using NAD+/NADH as a cofactor.[supplied by OMIM]

Database

Research Area

DATA IMAGES
Anti-PHGDH antibody [N1N2], N-term used in Western Blot (WB). GTX101948

GTX101948 WB Image

Wild-type (WT) and PHGDH knockout (KO) HeLa cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PHGDH antibody [N1N2], N-term (GTX101948) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PHGDH antibody [N1N2], N-term used in Immunoprecipitation (IP). GTX101948

GTX101948 IP Image

Immunoprecipitation of PHGDH protein from 293T whole cell extracts using 5 μg of PHGDH antibody [N1N2], N-term (GTX101948).
Western blot analysis was performed using PHGDH antibody [N1N2], N-term (GTX101948).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-PHGDH antibody [N1N2], N-term used in IHC (Frozen sections) (IHC-Fr). GTX101948

GTX101948 IHC-Fr Image

PHGDH antibody [N1N2], N-term detects PHGDH protein by immunohistochemical analysis.
Sample: Frozen-sectioned rat E13.5 brain.
Green: PHGDH stained by PHGDH antibody [N1N2], N-term (GTX101948) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-PHGDH antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101948

GTX101948 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using PHGDH(GTX101948) antibody at 1:500 dilution.

Anti-PHGDH antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX101948

GTX101948 IHC-P Image

Immunohistochemical analysis of paraffin-embedded PC13 xenograft, using PHGDH(GTX101948) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-PHGDH antibody [N1N2], N-term used in Western Blot (WB). GTX101948

GTX101948 WB Image

Sample(30 μg of whole cell lysate)
A:293T
B:A431(GTX27909)
C:HeLa S3(GTX14654)
7.5% SDS PAGE
GTX101948 diluted at 1:1000

Anti-PHGDH antibody [N1N2], N-term used in Western Blot (WB). GTX101948

GTX101948 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX101948 diluted at 1:1000

Anti-PHGDH antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX101948

GTX101948 IHC-P Image

PHGDH antibody [N1N2], N-term detects PHGDH protein at cytoplasm on mouse pancreas by immunohistochemical analysis.
Sample: Paraffin-embedded mouse pancreas.
PHGDH antibody [N1N2], N-term (GTX101948) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
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REVIEW

PHGDH antibody [N1N2], N-term Cat No. GTX101948

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 09-Oct-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : SK-N-DZ cells
Amount used : 20μg
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:1000, 25°C, 2Hr
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169