*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
293T , A431 , Jurkat , Raji , HeLa
Mouse, Bovine, Pig, Chimpanzee(>80% identity)
1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1 mg/ml (Please refer to the vial label for the specific concentration.)
Recombinant protein encompassing a sequence within the center region of human PHGDH. The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
phosphoglycerate dehydrogenase , 3-PGDH , 3PGDH , HEL-S-113 , NLS , NLS1 , PDG , PGAD , PGD , PGDH , PHGDHD , SERA
3-Phosphoglycerate dehydrogenase (PHGDH; EC 22.214.171.124) catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate, which is the first and rate-limiting step in the phosphorylated pathway of serine biosynthesis, using NAD+/NADH as a cofactor.[supplied by OMIM]
GTX101949 WB Image
Wild-type (WT) and PHGDH knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PHGDH antibody [N3C2], Internal (GTX101949) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX101949 IHC-P Image
Immunohistochemical analysis of paraffin-embedded human breast cancer, using PHGDH(GTX101949) antibody at 1:250 dilution.
GTX101949 WB Image
Sample (30 ug of whole cell lysate)
7.5% SDS PAGE
GTX101949 diluted at 1:10000
GTX101949 IHC-Fr Image
PHGDH antibody [N3C2], Internal detects PHGDH protein by immunohistochemical analysis.Sample: Frozen-sectioned rat E13.5 brain.Green: PHGDH stained by PHGDH antibody [N3C2], Internal (GTX101949) diluted at 1:250.Blue: Fluoroshield with DAPI (GTX30920).