Wild-type (WT) and PHGDH knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PHGDH antibody [N3C2], Internal (GTX101949) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded human breast cancer, using PHGDH(GTX101949) antibody at 1:250 dilution.

Sample (30 ug of whole cell lysate)
A: A431
B: Raji
7.5% SDS PAGE
GTX101949 diluted at 1:10000

PHGDH antibody [N3C2], Internal detects PHGDH protein by immunohistochemical analysis.
Sample: Frozen-sectioned rat E13.5 brain.
Green: PHGDH stained by PHGDH antibody [N3C2], Internal (GTX101949) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).