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PHGDH antibody [N3C2], Internal

KO/KD
Anti-PHGDH antibody [N3C2], Internal used in Western Blot (WB). GTX101949
Anti-PHGDH antibody [N3C2], Internal used in IHC (Frozen sections) (IHC-Fr). GTX101949
Anti-PHGDH antibody [N3C2], Internal used in Western Blot (WB). GTX101949
Anti-PHGDH antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX101949

Cat. No. GTX101949

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Fr

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all PHGDH products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:20000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Positive Control

A431 , Raji , HeLa

Predict Reactivity

Mouse, Bovine, Pig, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PHGDH. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11167012

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

phosphoglycerate dehydrogenase , 3-PGDH , 3PGDH , HEL-S-113 , NLS , NLS1 , PDG , PGAD , PGD , PGDH , PHGDHD , SERA

Background

3-Phosphoglycerate dehydrogenase (PHGDH; EC 1.1.1.95) catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate, which is the first and rate-limiting step in the phosphorylated pathway of serine biosynthesis, using NAD+/NADH as a cofactor.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-PHGDH antibody [N3C2], Internal used in Western Blot (WB). GTX101949

GTX101949 WB Image

KO/KD

Wild-type (WT) and PHGDH knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PHGDH antibody [N3C2], Internal (GTX101949) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-PHGDH antibody [N3C2], Internal used in IHC (Frozen sections) (IHC-Fr). GTX101949

GTX101949 IHC-Fr Image

PHGDH antibody [N3C2], Internal detects PHGDH protein by immunohistochemical analysis.
Sample: Frozen-sectioned rat E13.5 brain.
Green: PHGDH stained by PHGDH antibody [N3C2], Internal (GTX101949) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-PHGDH antibody [N3C2], Internal used in Western Blot (WB). GTX101949

GTX101949 WB Image

Sample (30 ug of whole cell lysate)
A: A431
B: Raji
7.5% SDS PAGE
GTX101949 diluted at 1:10000

Anti-PHGDH antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX101949

GTX101949 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using PHGDH(GTX101949) antibody at 1:250 dilution.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169