GeneTex
United States (US)

PHGDH antibody [N3C2], Internal

KO/KD
GTX101949_40604_20170818_WB_KO_watermark_19011506_855.jpg
GTX101949_40604_IHC_19011506_160.jpg
GTX101949_40604_WB_19011506_643.jpg
GTX101949_40604_20171106_IHC-Fr_R_19011506_193.jpg

Cat No. GTX101949

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, IHC-Fr
Reactivity Human, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:20000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Positive Control

293T , A431 , Jurkat , Raji , HeLa

Predict Reactivity

Mouse, Bovine, Pig, Chimpanzee(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PHGDH. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Phosphoglycerate Dehydrogenase,3-Pgdh,3Pgdh,Hel-S-113,Nls,Nls1,Pdg,Pgad,Pgd,Pgdh,Phgdhd,Sera,Phgdh

Background

3-Phosphoglycerate dehydrogenase (PHGDH; EC 1.1.1.95) catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate, which is the first and rate-limiting step in the phosphorylated pathway of serine biosynthesis, using NAD+/NADH as a cofactor.[supplied by OMIM]

Database

Research Area

DATA IMAGES
GTX101949_40604_20170818_WB_KO_watermark_19011506_855.jpg

GTX101949 WB Image

KO/KD

Wild-type (WT) and PHGDH knockout (KO) HeLa cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PHGDH antibody [N3C2], Internal (GTX101949) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
CRISPR knockout cell lines generated by www.edigene.com

GTX101949_40604_IHC_19011506_160.jpg

GTX101949 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using PHGDH(GTX101949) antibody at 1:250 dilution.

GTX101949_40604_WB_19011506_643.jpg

GTX101949 WB Image

Sample (30 ug of whole cell lysate)
A: A431
B: Raji
7.5% SDS PAGE
GTX101949 diluted at 1:10000

GTX101949_40604_20171106_IHC-Fr_R_19011506_193.jpg

GTX101949 IHC-Fr Image

PHGDH antibody [N3C2], Internal detects PHGDH protein by immunohistochemical analysis.
Sample: Frozen-sectioned rat E13.5 brain.
Green: PHGDH stained by PHGDH antibody [N3C2], Internal (GTX101949) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169