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PHGDH antibody

Anti-PHGDH antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64499
Anti-PHGDH antibody used in Western Blot (WB). GTX64499
Anti-PHGDH antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64499
Anti-PHGDH antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64499

Cat. No. GTX64499

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all PHGDH products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 264-533 of human PHGDH (NP_006614.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

phosphoglycerate dehydrogenase , 3-PGDH , 3PGDH , HEL-S-113 , NLS , NLS1 , PDG , PGAD , PGD , PGDH , PHGDHD , SERA

Background

This gene encodes the enzyme which is involved in the early steps of L-serine synthesis in animal cells. L-serine is required for D-serine and other amino acid synthesis. The enzyme requires NAD/NADH as a cofactor and forms homotetramers for activity. Mutations in this gene have been found in a family with congenital microcephaly, psychomotor retardation and other symptoms. Multiple alternatively spliced transcript variants have been found, however the full-length nature of most are not known. [provided by RefSeq, Aug 2011]

Database

Research Area

DATA IMAGES

Anti-PHGDH antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64499

GTX64499 ICC/IF Image

ICC/IF analysis of L929 cells using GTX64499 PHGDH antibody.
Blue : DAPI
Dilution : 1:100

Anti-PHGDH antibody used in Western Blot (WB). GTX64499

GTX64499 WB Image

WB analysis of various sample lysates using GTX64499 PHGDH antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-PHGDH antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64499

GTX64499 ICC/IF Image

ICC/IF analysis of H9C2 cells using GTX64499 PHGDH antibody.
Blue : DAPI
Dilution : 1:100

Anti-PHGDH antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64499

GTX64499 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX64499 PHGDH antibody.
Blue : DAPI
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399