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PHKA1 antibody [C2C3], C-term

Anti-PHKA1 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109401
Anti-PHKA1 antibody [C2C3], C-term used in Western Blot (WB). GTX109401
Anti-PHKA1 antibody [C2C3], C-term used in Western Blot (WB). GTX109401
Anti-PHKA1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX109401

Cat. No. GTX109401

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all PHKA1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

137 kDa. ( Note )

Positive Control

HeLa , 293T

Predict Reactivity

Rabbit, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human PHKA1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951219

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

phosphorylase kinase regulatory subunit alpha 1 , PHKA

Cellular Localization

Cell membrane; Lipid-anchor; Cytoplasmic side

Background

The PHKA1 gene encodes the alpha subunit of muscle phosphorylase kinase (EC 2.7.1.38), a key regulatory enzyme of glycogen metabolism. Phosphorylase kinase consists of 4 copies of an alpha-beta-gamma-delta tetramer. The alpha, beta (PHKB; MIM 172490), and gamma (PHKG1; MIM 172470 and PHKG2; MIM 172471) subunits have several isoforms; the delta subunit is calmodulin (CALM1; MIM 114180). PHKA2 (MIM 306000) encodes the alpha subunit of liver-specific phosphorylase kinase and is also located on the X chromosome.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-PHKA1 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109401

GTX109401 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using PHKA1(GTX109401) antibody at 1:200 dilution.

Anti-PHKA1 antibody [C2C3], C-term used in Western Blot (WB). GTX109401

GTX109401 WB Image

PHKA1 antibody detects PHKA1 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with PHKA1 antibody (GTX109401) diluted by 1:500.

Anti-PHKA1 antibody [C2C3], C-term used in Western Blot (WB). GTX109401

GTX109401 WB Image

Sample (30 ug of whole cell lysate)
A: HeLa
7.5% SDS PAGE
GTX109401 diluted at 1:1000

Anti-PHKA1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX109401

GTX109401 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using PHKA1(GTX109401) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Jin-Ho Koh et al. Nat Commun 2022; 13 (1) : 2324 Enhancement of anaerobic glycolysis - a role of PGC-1α4 in resistance exercise.
Application : WB
Reactivity : Mouse
Kim SH et al. J Physiol 2015; 593 (3) : 635-43 PGC-1α mediates a rapid, exercise-induced downregulation of glycogenolysis in rat skeletal muscle.
Application : WB
Reactivity : Rat
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169