GeneTex
United States (US)

PHKA2 antibody [C3], C-term

GTX108342_39834_IHC_19011507_217.jpg
GTX108342_WB_19011507_443.jpg

Cat No. GTX108342

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

138 kDa. ( Note )

Positive Control

HepG2
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PHKA2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Phosphorylase Kinase Regulatory Subunit Alpha 2,Gsd9A,Phk,Pyk,Pykl,Xlg,Xlg2,Phka2

Cellular Localization

Cell membrane; Lipid-anchor; Cytoplasmic side

Background

Phosphorylase b kinase catalyzes the phosphorylation of serine in certain substrates, including troponin I. The alpha chain may bind calmodulin.

Database

Research Area

DATA IMAGES
GTX108342_39834_IHC_19011507_217.jpg

GTX108342 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using PHKA2(GTX108342) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX108342_WB_19011507_443.jpg

GTX108342 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
5% SDS PAGE
GTX108342 diluted at 1:500

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169